Analysis of lifespan regulation mediated by calcium signaling in yeast
| Project/Area Number |
24580142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
MIZUNUMA Masaki 広島大学, 先端物質科学研究科, 准教授 (10343295)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 老化 / 寿命 / カルシウム / 細胞周期 / 酵母 |
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| Outline of Final Research Achievements |
In eukaryotic cells, the calcium ion (Ca2+) is involved in the regulation of various cellular functions as a signal molecule. We have conducted a life span analysis using all identified scz mutants of yeast. We measured the CLS (the length of time that a mother cell can survive in a non-dividing, quiescence-like state) of scz1~14 mutants. Some mutants extended CLS. One of the long-lived mutants was the cnb1/scz4 strain. The CNB1 encodes the regulatory subunit of CN. We are currently investigating how the CN affects life span. On the other hand, we found that a mutation of S-adenosylhomocysteine (SAH) hydrolase, sah1-1/scz7, affected cell growth and CLS. Thus, we have isolated the long-lived mutant by screening for the suppression of the growth defect of the sah1-1 mutant. This mutation was named SSG1. The SSG1 single mutant was shown to have a longer CLS than a wild-type yeast. Therefore, the Ssg1 protein is predicted to be a factor in longevity. We are investing this phenotype.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Late-maturing cooking rice Sensyuraku has excellent properties, equivalent to sake rice, for high-quality sake brewing.2014
Author(s)
Anzawa Y, Satoh K, Satoh Y, Ohno S, Watanabe T, Katsumata K, Kume K, Watanabe K, Mizunuma M, Hirata D.
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Journal Title
Biosci Biotechnol Biochem.
Volume: 78
Issue: 11
Pages: 1954-1962
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Evidence of Antagonistic Regulation of Restart from G<sub>1</sub> Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast2013
Author(s)
Masaki Mizunuma, Takafumi Ogawa, Tetsuya Koyama, Atsunori Shitamukai, Ryohei Tsubakiyama, Tadamasa Komaruyama, Toshinaga Yamaguchi, Kazunori Kume and Dai Hirata
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 10
Pages: 2002-2007
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Polishing Properties of Sake Rice <i>Koshitanrei</i> for High-Quality Sake Brewing2013
Author(s)
Anzawa Y, Nabekura Y, Satoh K, Satoh Y, Ohno S, Watanabe T, Kaneoke M, Kume K, Mizunuma M, Watanabe K, Katsumata K, Hirata D
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 10
Pages: 2160-2165
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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