| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In eukaryotic cells, the calcium ion (Ca2+) is involved in the regulation of various cellular functions as a signal molecule. We have conducted a life span analysis using all identified scz mutants of yeast. We measured the CLS (the length of time that a mother cell can survive in a non-dividing, quiescence-like state) of scz1~14 mutants. Some mutants extended CLS. One of the long-lived mutants was the cnb1/scz4 strain. The CNB1 encodes the regulatory subunit of CN. We are currently investigating how the CN affects life span. On the other hand, we found that a mutation of S-adenosylhomocysteine (SAH) hydrolase, sah1-1/scz7, affected cell growth and CLS. Thus, we have isolated the long-lived mutant by screening for the suppression of the growth defect of the sah1-1 mutant. This mutation was named SSG1. The SSG1 single mutant was shown to have a longer CLS than a wild-type yeast. Therefore, the Ssg1 protein is predicted to be a factor in longevity. We are investing this phenotype.
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