| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, by utilizing C2C12 contractile model, we attempted to mimic different types of exercise, such as vigorous exercise, and analyzed exercise-type dependent changes in cellular response, such as production of myokines. Differentiated C2C12 myotubes were subjected to different voltage, pulse frequency, duration of electrical pulse stimulation (EPS) to induce contraction, thereby analyzed secreted proteins by membrane antibody arrays. Several novel myokines, which were induced by vigorous exercise, were successfully identified by this method, particularly, we found two myokines, including RANTES, were significantly reduced by vigorous exercise. In addition, we found exercise-dependent AMP kinase activation was sufficient for reducing secreted RANTES levels. In conclusion, we identified RANTES as a novel myokine that secretion is reduced by vigorous skeletal muscle cell contraction, and this reduction is mediated by AMP kinase pathway.
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