Development of controlling system of cell differentiation/life by proteolysis resistant cell-permeable protein
| Project/Area Number |
24580148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Taiichirou 日本大学, 生物資源科学部, 教授 (20187834)
HANAZAWA Shigemasa 日本大学, 生物資源科学部, 教授 (60060258)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 分解耐性 / スタビロン / 膜透過性タンパク質 / 細胞分化制御 / 細胞機能調節 / Stabilon / iPS細胞 / 制御性T細胞 / Treg / 肝細胞分化 / Foxp3 / HNF3β / Sox17 / 細胞膜透過性 / 細胞分化調節 / RAR / 細胞膜透過性タンパク質 / Treg分化 / 長寿 / Sirt / タンパク質分解耐性 |
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| Outline of Final Research Achievements |
I established the expression system of the proteolysis resistant cell-permeable differentiation control factor (Yamanaka Factor [Glis1, Sox2, Kof4, Oct4], RARα, Foxp3, Sox17, HNF3β ). These many factors showed good expression in Escherichia coli and maintained transcription activity. In addition, I found that a fusion position of the proteolysis-resistant motif stabilon was effective at all positions. Furthermore, unlike an old stabilon motif, I developed a proteolysis-resistant motif of the absent new sequence in the creatures.
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Report
(4 results)
Research Products
(11 results)
