| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
We succeeded to construct the serine racemase gene-disruption mutant and overexpressed mutant of Arabidopsis thaliana. By using these mutants and wild-type strain of Arabidopsis thaliana, we evaluated the effects of various D- and L- amino acids on germination and growth of these strains. In case of D-serine, the serine racemase gene overexpressed mutant showed higher tolerant than other strains. This probably attributed to the D-serine degradation activity of serine rasemase. In case of L-serine, both serine racemase gene-disruption mutant and overexpressed mutant showed higher tolerant than wild-type strain.
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