Studies on jasmonic acid signalling for plants defense induction

• Project/Area Number: 24580165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Akita Prefectural University
• Principal Investigator: TAMOGAMI Shigeru 秋田県立大学, 生物資源科学部, 教授 (70315589)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 植物ホルモン / ジャスモン酸 / メチルジャスモン酸 / ジャスモノイルイソロイシン / 防御反応 / 揮発性化合物 / テルペン / 食害 / 自己防御 / LC-MS/MS / ヒナタイノコズチ / 揮発性物質 / デノボ合成 / 重水素 / ラベル化反応 / 植物揮発性化合物

Outline of Final Research Achievements

Plant hormones function as signalling compounds which defend against environmental stresses or herbivore damage. Especially, jasmonic acid (JA) and its related metabolites work in the herbivore damaged leaves. Recent studies have shown that JA its self is inactive, but its isoleucine conjugate (JA-Ile) is active. It has been known that herbivore damage can activate plants defense at distant tissues, and signalling compounds may control the whole-plant defense induction. Highly mobile MeJA can be a candidate as the whole-plant defensive signalling molecule. In this study, transportation of MeJA and its activation into JA-Ile in the distant tissues (upper leaves) were investigated using an experimental mode plant Achyranthes bidentata (Ox knee). Exogenously applied MeJA was transported into upper distant leaves and activated into JA-Ile. Induced defensive terpene metabolites in the distant leaves were shown to be de-novo metabolites.

Research Products

• [Journal Article] Methyl jasmonate elicits the production of methyl (E)-2-hexenoate (2015)
  • Author(s): Tamogami, S., Noge, K., Agrawal, G. K., Rakwal, R.
  • Journal Title: FEBS Letters Volume: 589 Issue: 3 Pages: 390-395
  • DOI: 10.1016/j.febslet.2014.12.025
  • Peer Reviewed / Open Access
• [Journal Article] Deuterium labeling for investigating de novo synthesis of terpene volatiles in Achyranthes bidentata. (2013)
  • Author(s): Tamogami, S., Noge, K., Abe, M., Agrawal, G. K., and Rakwal, R
  • Journal Title: Biotechnology Letters Volume: 35 Issue: 8 Pages: 1247-1252
  • DOI: 10.1007/s10529-013-1201-y
  • Peer Reviewed
• [Journal Article] Methyl jasmonate is transported to distal leaves via vascular process metabolizing itself into JA-Ile and triggering VOCs emission as defensive metabolites (2012)
  • Author(s): Shigeru Tamogami, Koji Noge, Makoto Abe, Ganesh Kumar Agrawal, Randeep Rakwal
  • Journal Title: Plant Signaling & Behavior Volume: 7 Issue: 11 Pages: 1378-1381
  • DOI: 10.4161/psb.21762
  • Peer Reviewed
• [Presentation] ヒナタイノコズチでの(Z)-3-ヘキセノールの代謝と異性化 (2015)
  • Author(s): 清水理未、野下浩二、阿部誠、田母神繁
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学農学部
  • Year and Date: 2015-03-28
• [Presentation] ヒナタイノコズチ葉におけるmethyl(E)-2-hexenoateの生合成経路 (2015)
  • Author(s): 田母神繁、野下浩二、阿部誠、ガネシ・アルラワール、ランディープ・ラクワール
  • Organizer: 日本農薬学会第40回大会
  • Place of Presentation: 玉川大学農学部
  • Year and Date: 2015-03-20
• [Presentation] メチルジャスモン酸により放出されるヒナタイノコズチ C6 化合物の構造と生合成
  • Author(s): 田母神繁 野下浩二 阿部誠
  • Organizer: 日本農薬学会
  • Place of Presentation: 京都大学 農学部
• [Presentation] 植物シグナル物質メチルジャスモン酸の移行・代謝・防御反応の誘導
  • Author(s): 田母神繁，野下浩二，阿部誠
  • Organizer: 日本農芸化学会東北支部第147回大会
  • Place of Presentation: 弘前大学農学部
• [Presentation] 植物シグナル物質メチルジャスモン酸の機能解析
  • Author(s): 田母神繁，野下浩二，阿部誠，ガネシュ クマール アグラワール，ランディープ ラクワール
  • Organizer: 日本農薬学会第38回大会
  • Place of Presentation: 筑波大学
• [Book] Springer Protocols. Methods in Molecular Biology. Rice Protocols. Chapter 13: Quantification of Jasmonic and salicylic acids in rice seedling leaves (2013)
  • Author(s): Cho, K., Han, O., Tamogami, S. Shibato, J., Kubo, A., Agrawal, G.K., Rakwal R.
  • Total Pages: 15
  • Publisher: Humana Press (Springer Group)