Elucidation of the mechanism of matriptase activation and exploration of food components that control the activation.

• Project/Area Number: 24580178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: TSUZUKI Satoshi 京都大学, (連合)農学研究科(研究院), 助教 (50283651)
• Co-Investigator(Renkei-kenkyūsha): INOUYE Kuniyo (10223249)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 酵素 / 食品 / 遺伝子 / バイオテクノロジー / 栄養学

Outline of Final Research Achievements

Matriptase is a transmembrane serine protease. In this study, we found that low-density lipoprotein receptor class A domain repeat in the stem domain of matriptase acts to enhance its zymogen activity and that some kinds of divalent cations are required for the expression of zymogen activity. In addition, we obtained evidence supporting that the activation of matriptase zymogen occurs by a mechanism requiring the interaction of the two molecules (transactivation).

Research Products

• [Journal Article] A recombinant matriptase causes an increase in caspase-3 activity in a small-intestinal epithelial IEC-6 line cultured on fibronectin-coated plates (2014)
  • Author(s): Seiya Mochida , Satoshi Tsuzuki , Kuniyo Inouye & Tohru Fushiki
  • Journal Title: Cytotechnology Volume: 66 Issue: 3 Pages: 357-363
  • DOI: 10.1007/s10616-013-9582-2
  • Peer Reviewed
• [Journal Article] Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation (2013)
  • Author(s): Inouye K, Tomoishi M, Yasumoto M, Miyake Y, Kenji Kojima, Tsuzuki S, Fushiki T
  • Journal Title: Journal of Biochemistry Volume: 153 Issue: 1 Pages: 51-61
  • DOI: 10.1093/jb/mvs118
  • NAID: 120005373084
  • Peer Reviewed
• [Presentation] マトリプターゼ前駆体の酵素活性に対するLDL受容体クラスAドメインの役割解明
  • Author(s): 友石満里絵、都築巧、安元誠、伏木亨、兒島憲二、井上國世
  • Organizer: 2012年度 日本農芸化学会 関西支部大会（第476回講演会）
  • Place of Presentation: 京都学園大学
• [Presentation] マトリプターゼLDL受容体クラスAドメインの意義解明
  • Author(s): 友石満里絵、都築巧、安元誠、伏木亨、兒島憲二、井上國世
  • Organizer: 第59回日本生化学会近畿支部例会
  • Place of Presentation: 京都大学 宇治おうばくプラザ