Production of aminosugar-containing hetero-oligosaccharides modulating gut microflora that promotes health and longevity

• Project/Area Number: 24580179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kinki University
• Principal Investigator: ASHIDA Hisashi 近畿大学, 生物理工学部, 教授 (40379087)
• Co-Investigator(Kenkyū-buntansha): FUKUZAWA Hideya 京都大学, 大学院生命科学研究科, 教授 (30183924)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ビフィズス菌 / プレバイオティクス / グリコシダーゼ / 腸内細菌 / バイオマス / ヘテロオリゴ糖

Outline of Final Research Achievements

Galacto-N-biose, an aminosugar-containing hetero-disaccharides, is one of the most promising bifidogenic factors, which is specifically assimilated by bifidobacteria. We produced galacto-N-biose from mucin-type O-glycans of cheese whey glycomacropeptide and gastric mucin as substrates by using the combination of various glycosidases from bifidobacteria.

Research Products

• [Journal Article] α-N-Acetylglucosaminidase from Bifidobacterium bifidum specifically hydrolyzes α-linked N-acetylglucosamine at nonreducing terminus of O-glycan on gastric mucin (2015)
  • Author(s): 芦田久ら
  • Journal Title: Appl Microbiol Biotechnol. Volume: 99 Issue: 9 Pages: 3941-3948
  • DOI: 10.1007/s00253-014-6201-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural analysis of cerebrosides from Aspergillus fungi: the existence of galactosylceramide in A. oryzae (2014)
  • Author(s): Tani Y, Amaishi Y, Funatsu T, Ito M, Itonori S, Hata Y, Ashida H, Yamamoto K
  • Journal Title: Biotechnology Letters Volume: 36 Issue: 12 Pages: 2507-2513
  • DOI: 10.1007/s10529-014-1631-1
  • Peer Reviewed
• [Journal Article] β-Glucuronidase from Lactobacillus brevis useful for baicalin hydrolysis belongs to glycoside hydrolase family 30 (2014)
  • Author(s): Sakurama H, Kishino S, Uchibori Y, Yonejima Y, Ashida H, Kita K, Takahashi S, Ogawa J
  • Journal Title: Applied Microbiology and Biotechnology Volume: 98 Issue: 9 Pages: 4021-4032
  • DOI: 10.1007/s00253-013-5325-8
  • Peer Reviewed
• [Journal Article] Crystal structures of a glycoside hydrolase family 20 lacto-N-biosidase from Bifidobacterium bifidum (2013)
  • Author(s): 伊藤佑、芦田久、伏信進矢ら
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 17 Pages: 11795-11806
  • DOI: 10.1074/jbc.m112.420109
  • Peer Reviewed
• [Journal Article] Deficiency of α-glucosidase I alters glycoprotein glycosylation and lifespan in Caenorhabditis elegans (2013)
  • Author(s): Katoh T, Takase J, Tani Y, Amamoto R, Aoshima N, Tiemeyer M, Yamamoto K, Ashida H
  • Journal Title: Glycobiology Volume: 23 Issue: 10 Pages: 1142-1151
  • DOI: 10.1093/glycob/cwt051
  • Peer Reviewed
• [Journal Article] Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression (2013)
  • Author(s): 櫻間晴子、芦田久ら
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 35 Pages: 25194-25206
  • DOI: 10.1074/jbc.m113.484733
  • Peer Reviewed
• [Journal Article] Bifidobacterial α-galactosidase with unique carbohydrate-binding module specifically acts on blood group B antigen (2013)
  • Author(s): Takura Wakinaka, Masashi Kiyohara, Shin Kurihara, Akiko Hirata, Thida Chaiwangsri, Takayuki Ohnuma, Tamo Fukamizo, Takane Katayama, Hisashi Ashida, and Kenji Yamamoto
  • Journal Title: Glycobiology Volume: 23 Issue: 2 Pages: 232-240
  • DOI: 10.1093/glycob/cws142
  • Peer Reviewed
• [Journal Article] Anτ-N-Acetylgalactosaminidase from Infant-associated Bifid bacteria Belonging to a Novel Glycoside Hydrolase Family 129 is Implicated in an Alternative Mucin Degradation Pathway (2012)
  • Author(s): Masashi Kiyohara, Takashi Nakatomi, Shin Kurihara, Shinya Fushinobu, Hideyuki Suzuki, Tomonari Tanaka, Shin-ichiro Shoda, Takane Katayama, Kenji Yamamoto, Hisashi Ashida
  • Journal Title: J. Biol. Chem Volume: 287 Issue: 1 Pages: 693-700
  • DOI: 10.1074/jbc.m111.277384
  • Peer Reviewed
• [Presentation] Sda抗原を分解するビフィズス菌由来のバイファンクショナル酵素 (2015)
  • Author(s): 島田良美、香川昂雅、普家崇史、堀西朝子、尾﨑嘉彦、芦田 久
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 梅ポリフェノールのマウス腸内細菌に及ぼす影響 (2014)
  • Author(s): 島田良美、香川昴雅、晋家崇史、尾﨑嘉彦、芦田 久
  • Organizer: 第12回 果実酒・果実飲料と健康に関する研究会
  • Place of Presentation: 和歌山県立医科大学（和歌山県和歌山市）
  • Year and Date: 2014-11-29
• [Presentation] ムチン型糖鎖に作用するグリコシダーゼ ～土壌微生物から腸内細菌へ～ (2014)
  • Author(s): 芦田 久
  • Organizer: 日本応用糖質科学会 第39回近畿支部会・講演会
  • Place of Presentation: 近畿大学会館（大阪府大阪市）
  • Year and Date: 2014-11-15
  • Invited
• [Presentation] ムチンの糖鎖に作用するビフィズス菌の新奇グリコシダーゼ (2014)
  • Author(s): 芦田 久
  • Organizer: 食品酵素化学研究会第14回学術講演会
  • Place of Presentation: 大阪府立大学（大阪府大阪市）
  • Year and Date: 2014-08-30
• [Presentation] Screening and analysis of useful glycosidases for production of bifidogenic factors (2014)
  • Author(s): Hisashi Ashida, Yoshimi Shimada, Yoshihisa Funeno, Masayuki Kubota, Thida Chaiwangsri, Toshihiko Katoh, Takashi Koyanagi, Hisanori Tamaki, Kenji Yamamoto and Takane Katayama
  • Organizer: The 1st Joint Seminar. New Core to Core Program. A. Advanced Research Networks on "Establishment of an International Research Core for New Bio-Research Fields with Microbes from Tropical Areas"
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2014-08-10 – 2014-08-11
• [Presentation] ビフィズス菌由来の新規ラクト-N-ビオシダーゼの同定と機能解析 (2014)
  • Author(s): 櫻間晴子、清原正志、芦田 久、北岡本光、高橋里美、山本憲二、片山高嶺
  • Organizer: 第15回 関西グライコサイエンスフォーラム
  • Place of Presentation: 大阪市立大学（大阪府大阪市）
  • Year and Date: 2014-05-24
• [Presentation] 植物性多糖に作用するBifidobacterium longum由来の酵素の解析 (2013)
  • Author(s): 芦田 久，北村有里奈，竹内涼子
  • Organizer: 平成25年度 日本農芸化学会大会
  • Place of Presentation: 東北大学（宮城県）
• [Presentation] Analyses of metabolic pathway of oligosaccharides and its related enzymes in useful lactic acid bacteria (2012)
  • Author(s): Thida Chaiwangsri, Masashi Kiyohara, Hisashi Ashida, Takane Katayama, Kenji Yamamoto
  • Organizer: The final joint seminar of Asian Core Program (2008-2013) on "Capacity Building and Development of Microbial Potential and Fermentation Technology towards New Era"
  • Place of Presentation: 下関海洋メッセ（山口県）
• [Presentation] Bifidobacterium longum subsp. longum JCM 1217 由来GH31グリコシダーゼの解析 (2012)
  • Author(s): 竹内涼子、福澤秀哉、芦田 久
  • Organizer: 第85回 日本生化学会大会
  • Place of Presentation: マリンメッセ福岡（福岡県）
• [Presentation] Bifidobacterium longum subsp. longum JCM 1217 由来の酵素GH43グリコシダーゼの解析 (2012)
  • Author(s): 北村有里奈、福澤秀哉、芦田 久
  • Organizer: 第85回 日本生化学会大会
  • Place of Presentation: マリンメッセ福岡（福岡県）
• [Presentation] 食品由来オートファジー誘導物質の寿命への効果
  • Author(s): 芦田 久
  • Organizer: 第11回果実酒・果実飲料と健康に関する研究会
  • Place of Presentation: 近畿大学生物理工学部（和歌山県紀の川市）
  • Invited
• [Presentation] グルコサミンによるオートファジー誘導と寿命への効果
  • Author(s): 芦田 久
  • Organizer: 平成25年度 日本応用糖質科学会東日本支部シンポジウム
  • Place of Presentation: 東京大学農学部弥生講堂（東京都文京区）
  • Invited
• [Presentation] Glucosamine as an Autophagy Inducer
  • Author(s): Hisashi Ashida
  • Organizer: 3th International Conference of Functional Food Center
  • Place of Presentation: 京都府立医科大学（京都府京都市上京区）
  • Invited
• [Presentation] ユニークな糖結合モジュールCBM51を含むビフィズス菌のGH110 α-ガラクトシダーゼ
  • Author(s): 脇中琢良、清原正志、大沼貴之、深溝 慶、山本憲二、芦田 久
  • Organizer: 第13回 関西グライコサイエンスフォーラム
  • Place of Presentation: 大阪市立大学（大阪府）
• [Book] Glycobiology of the Nervous System (2014)
  • Author(s): Ashida H, Li YT
  • Total Pages: 584
  • Publisher: Springer
• [Remarks] 芦田久－研究者－ReaD & Researchmap
  • URL: http://researchmap.jp/ashida/