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Screening of collagen-degrading enzyme from marine organisms and its application to meat tenderization

Research Project

Project/Area Number 24580186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionKagawa University

Principal Investigator

OGAWA Masahiro  香川大学, 農学部, 教授 (10398034)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsコラーゲン / 食肉軟化酵素 / プロテアーゼ / パパイン / テクスチャー / 塩漬液 / コラーゲン分解酵素 / 食肉軟化 / 培養方法 / スクリーニング / 耐塩性
Outline of Final Research Achievements

A method that is capable of tenderizing tough meat without losing meat-specific texture is required in meat industry. In this study, we performed screening of collagen-degrading enzyme (CDE) from marine organisms including sea animals and bacteria and evaluated the effect of CDE on the physical property of meat. Sea animal guts (yellowtail and scallop) and 89 bacterial strains adhering to fish skins showed collagen-degrading activity. Of all the marine organisms tested, Pseudomonas sp. SF10 isolated from flat fish and Shewanella sp. JS51 isolated from sea bass possessed CDEs with highest collagen-degrading activity. SF10-CDE was injected into beef chunks. The CDE-treated meat which was incubated at 4 C for 5 days was significantly tenderer than untreated meat.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) (of which Invited: 2 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Thermal Stability of Chicken Keel Bone Collagen2014

    • Author(s)
      J.N. Losso and M. Ogawa
    • Journal Title

      Journal of Food Biochemistry

      Volume: 39 Issue: 3 Pages: 345-351

    • DOI

      10.1111/jfbc.12059

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transepithelial transports of rare sugar D‑psicose in human intestine2013

    • Author(s)
      Hishiike, T., Ogawa, M., Hayakawa, S., Nakajima, D., O’Charoen, S., Ooshima, H., and Sun, Y.
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 61 Issue: 30 Pages: 7381-7386

    • DOI

      10.1021/jf401449m

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Meat tenderization by novel bacterial collagenases2014

    • Author(s)
      Masahiro Ogawa
    • Organizer
      International Symposium on Food and Agro-biodiversity (ISFA) 2014
    • Place of Presentation
      セマラン市(インドネシア)
    • Year and Date
      2014-09-16 – 2014-09-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Transepithelial Transports of Rare Sugar D‑Psicose in Human Intestine2014

    • Author(s)
      Masahiro Ogawa and Shigeru Hayakawa
    • Organizer
      国際希少糖学会 第六回国際シンポジウム
    • Place of Presentation
      かがわ国際会議場(香川県高松市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ニワトリ卵白塩基性タンパク質のアルカリ処理による構造変化

    • Author(s)
      丹羽貴大,赤澤隆志,早川茂,小川雅廣
    • Organizer
      日本農芸化学会中四国支部第38回講演会
    • Place of Presentation
      香川大学農学部(香川県木田郡三木町)
    • Related Report
      2013 Research-status Report
  • [Presentation] 魚類付着細菌由来コラーゲン分解酵素のスクリーニングおよび同酵素の食肉軟化作用

    • Author(s)
      小川雅廣,河尻芙生子,竹内千智,早川茂, 安倍恵,田邉学,中出浩二,東久邇眞彦,小齊喜一
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      藤女子大学(北海道)
    • Related Report
      2012 Research-status Report
  • [Book] 農学入門 ― 食料・生命・環境科学の魅力 ―, 安田弘法・中村宗一郎・太田寛行・橘 勝康・生源寺真一編著, “第6章 おいしさの科学”2013

    • Author(s)
      橘勝康,小川雅廣
    • Total Pages
      438
    • Publisher
      養賢堂
    • Related Report
      2013 Research-status Report
  • [Remarks] 小川研究室研究紹介

    • URL

      http://www.ag.kagawa-u.ac.jp/?research=%E5%B0%8F%E5%B7%9D%E9%9B%85%E5%BB%A3%E3%80%80%EF%BC%88%E9%A3%9F%E5%93%81%E3%82%BF%E3%83%B3%E3%83%91%E3%82%AF%E8%B3%AA%E5%8C%96%E5%AD%A6%EF%BC%89

    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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