| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The effects of collagen from jellyfish on the innate immune response, especially on the activity of macrophages were evaluated. The phagocytosis of macrophage was enhanced by collagen. Jellyfish collagen also stimulated tumour necrosis factor-α and interleukin-6 production of macrophages as the result of elevated gene expression levels of these cytokines. Oral administration of collagens elevated cytokine production by peritoneal macrophages in mice. On the contrary, collagen suppressed gene expression of PPARγ1 in macrophages. It is supposed from this result that collagen may stimulate the cytokine production by suppressing the PPARγ1 expression. The activity of jellyfish collagen was cancelled by TLR4 inhibitor. Moreover, jellyfish collagen stimulated phosphorylation of IκBα, promoted translocation of NF-κB, and activated JNK. Jellyfish collagen also activated and induced maturation of mouse bone marrow-derived dendritic cells.
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