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Effect of collagen on the innate immune system.

Research Project

Project/Area Number 24580188
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionEhime University

Principal Investigator

SUGAHARA Takuya  愛媛大学, 農学部, 教授 (00263963)

Research Collaborator MATSUMOTO Yoshiteru  愛媛県認定漁業士協同組合, 理事長
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsコラーゲン / 自然免疫 / マクロファージ / 樹状細胞 / サイトカイン産生 / TLR4 / JNK / ヒラメ滑走細菌 / ブリ類結節症
Outline of Final Research Achievements

The effects of collagen from jellyfish on the innate immune response, especially on the activity of macrophages were evaluated. The phagocytosis of macrophage was enhanced by collagen. Jellyfish collagen also stimulated tumour necrosis factor-α and interleukin-6 production of macrophages as the result of elevated gene expression levels of these cytokines. Oral administration of collagens elevated cytokine production by peritoneal macrophages in mice. On the contrary, collagen suppressed gene expression of PPARγ1 in macrophages. It is supposed from this result that collagen may stimulate the cytokine production by suppressing the PPARγ1 expression. The activity of jellyfish collagen was cancelled by TLR4 inhibitor. Moreover, jellyfish collagen stimulated phosphorylation of IκBα, promoted translocation of NF-κB, and activated JNK. Jellyfish collagen also activated and induced maturation of mouse bone marrow-derived dendritic cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (4 results) Remarks (6 results)

  • [Journal Article] Jellyfish collagen stimulates maturation of mouse bone marrow-derived dendritic cells.2015

    • Author(s)
      Putra A.B.N., Nishi K., Shiraishi R., Doi M. and Sugahara T.
    • Journal Title

      Journal of Functional Foods

      Volume: 14 Pages: 308-317

    • DOI

      10.1016/j.jff.2015.02.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Jellyfish collagen stimulates production of TNF-α and IL-6 by J774.1 cells through activation of NF-kappaB and JNK via TLR4 signaling pathway.2014

    • Author(s)
      Putra A.B.N., Nishi K., Shiraishi R. Moi M. and Sugahara T.
    • Journal Title

      Molecular Immunology

      Volume: 58 Issue: 1 Pages: 32-37

    • DOI

      10.1016/j.molimm.2013.11.003

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo2013

    • Author(s)
      Ika Dyah Kumalasari et al.
    • Journal Title

      Cytotechnology

      Volume: in press Issue: 1 Pages: 75-85

    • DOI

      10.1007/s10616-013-9539-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of collagens from jellyfish and bovine Achilles tendon on the activity of J774.1 and mouse peritoneal macrophage cells2012

    • Author(s)
      Agus Budiawan Naro Putra et al.
    • Journal Title

      Journal of Functional Foods

      Volume: 4 Issue: 2 Pages: 504-512

    • DOI

      10.1016/j.jff.2012.02.011

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Collagen from jellyfish stimulates mouse bone marrow-derived dendritic cells.2015

    • Author(s)
      Putra A.B.N., Nishi K., Shiraishi R., Doi M. and Sugahara T.
    • Organizer
      12th Asian Congress of Nutrition (ACN2015 )
    • Place of Presentation
      横浜(日本)
    • Year and Date
      2015-05-14 – 2015-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Immunostimulatory effects of jellyfish collagen on mouse bone marrow-derived dendritic cells.2014

    • Author(s)
      Putra A.B.N., Nishi K., Shiraishi R., Doi M. and Sugahara T.
    • Organizer
      7th International Conference and Exhibition on Nutraceuticals and Functional Foods (ISNFF 2014)
    • Place of Presentation
      イスタンブール(トルコ)
    • Year and Date
      2014-10-14 – 2014-10-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Jellyfish collagen stimulates production of TNF-α and IL-6 by J774.1 cells through TLR4 signaling pathway.2014

    • Author(s)
      Agus Budiawan Naro Putra, Kosuke Nishi, Ryusuke Shiraishi, Mihikaru Doi, Takuya Sugahara
    • Organizer
      International Conference and Exhibition on Nutraceuticals and Functional Foods (ISNFF2014)
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2013 Research-status Report
  • [Presentation] Studies on the immunostimulatory effects of jellyfish collagen on macrophage.2012

    • Author(s)
      Putra A.B.N., Morishige H., Nishimoto S., Nishi K., Shiraishi R., Doi M. and Sugahara T.
    • Organizer
      The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2012 Research-status Report
  • [Remarks] 愛媛大学農学部動物細胞工学研究室

    • URL

      http://web-amb.agr.ehime-u.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 愛媛大学農学部附属食品健康科学研究センター

    • URL

      http://fhsrc.agr.ehime-u.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 愛媛大学農学部動物細胞工学研究室

    • URL

      http://web-amb.agr.ehime-u.ac.jp/

    • Related Report
      2013 Research-status Report
  • [Remarks] 愛媛大学農学部附属食品健康科学研究センター

    • URL

      http://fhsrc.agr.ehime-u.ac.jp/

    • Related Report
      2013 Research-status Report
  • [Remarks] 愛媛大学農学部動物細胞工学研究室

    • URL

      http://web-amb.agr.ehime-u.ac.jp

    • Related Report
      2012 Research-status Report
  • [Remarks] 愛媛大学農学部附属食品健康科学研究センター

    • URL

      http://fhsrc.agr.ehime-u.ac.jp

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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