| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Eosinophils are multifunctional leukocytes involved in parasite elimination, evocation of allergic reactions, and regulation of adipose tissue. IL-5 is required for eosinophil survival; however, the in vivo mechanisms of eosinophil regulation are not fully understood. A transgenic (tg) mouse model with Il5 promoter-driven EGFP expression was established for detecting the IL-5-producing cells in vivo. The first part of the results demonstrate that innate lymphoid cells (ILCs) activate eosinophils in GAT. The blockage of IL-33R, on the other hand, did not impair EGFP+ ILC numbers but did impair eosinophil numbers in vivo. IL-33 was found to expand eosinophil numbers in CD90+ cell-depleted mice. The latter part of findings demonstrate that IL-33 directly activates eosinophils in GAT, and together with our other findings described above, our findings show that IL-33 has dual pathways via which it activates eosinophils in vivo: a direct activation pathway and a group 2 ILC-mediated pathway.
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