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Functional study of IL-5-producing cells in food allergy using GFP marking system

Research Project

Project/Area Number 24580196
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionDokkyo Medical University

Principal Investigator

MASAAKI Hashiguchi  獨協医科大学, 医学部, 准教授 (20372443)

Research Collaborator KOBAYASHI Ayano  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsアレルギー / IL-5 / IL-33 / 好酸球 / GFP / マーキング
Outline of Final Research Achievements

Eosinophils are multifunctional leukocytes involved in parasite elimination, evocation of allergic reactions, and regulation of adipose tissue. IL-5 is required for eosinophil survival; however, the in vivo mechanisms of eosinophil regulation are not fully understood. A transgenic (tg) mouse model with Il5 promoter-driven EGFP expression was established for detecting the IL-5-producing cells in vivo. The first part of the results demonstrate that innate lymphoid cells (ILCs) activate eosinophils in GAT. The blockage of IL-33R, on the other hand, did not impair EGFP+ ILC numbers but did impair eosinophil numbers in vivo. IL-33 was found to expand eosinophil numbers in CD90+ cell-depleted mice. The latter part of findings demonstrate that IL-33 directly activates eosinophils in GAT, and together with our other findings described above, our findings show that IL-33 has dual pathways via which it activates eosinophils in vivo: a direct activation pathway and a group 2 ILC-mediated pathway.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] IL-33 activates eosinophils of visceral adipose tissue both directly and via innate lymphoid cells2015

    • Author(s)
      Hashiguchi, M., Kashiwakura, Y., Kojima, H., Kobayashi, A., Kanno, Y., Kobata, T.
    • Journal Title

      Eur. J. Immunol.

      Volume: 45 Issue: 3 Pages: 876-885

    • DOI

      10.1002/eji.201444969

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Dual activation pathways of visceral adipose tissue eosinophils by interleukin-33 in vivo: innate lymphoid cell-mediated and direct actions2014

    • Author(s)
      Hashiguchi Masaaki, Kashiwakura Yuji, Kojima Hidefumi, Kanno Yumiko, Kobata Tetsuji
    • Organizer
      日本免疫学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2014-12-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] IL-5 is produced by a novel subset of innate lymphoid cell with phenotypes of Lin^- Thy1.2^low-int CD25^+ IL-33Rα^+ in Peyer’s patches

    • Author(s)
      Masaaki Hashiguchi, Yuji Kashiwakura, Yumiko Kanno, Hidefumi Kojima, Tetsuji Kobata
    • Organizer
      日本免疫学会学術集会
    • Place of Presentation
      幕張
    • Related Report
      2013 Research-status Report
  • [Presentation] NF-κB downregulates IL-5 production via upregulation of T-box proteins

    • Author(s)
      Masaaki Hashiguchi, Hidefumi Kojima, Yuji Kashiwakura, Yumiko Kanno, Tetsuji Kobata
    • Organizer
      日本免疫学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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