| Project/Area Number |
24580198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
UEHARA Mariko 東京農業大学, 応用生物科学部, 教授 (20211071)
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| Research Collaborator |
ADLERCREUTZ Herman University of Helsinki, Finland, Folkhälsan Research Center
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 大豆イソフラボン / equol / 鏡像異性体 / 難消化性糖質 / 骨粗鬆症 / フラクトオリゴ糖 |
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| Outline of Final Research Achievements |
Equol, a metabolite of isoflavone, is a chiral molecule that exists as the enantiomers (R)(+)-equol and (S)(-)-equol.To compare the effect of R-equol and S-equol on bone loss, ovariectomized (OVX) mice were used and each equol(0.5mg/day) were administered to the OVX mice. As natural selective estrogen receptor modulators (SERMs), the equol enantiomers inhibited bone loss, while minimizing the impact on the uterus. S-equol had stronger effects than R-equol in vivo. We performed transcriptome analysis to comprehensively understand the effects of equol enentiomers in mouse femur．DNA microarray analysis demonstrated that S-equol might down-regulate the expression of osteoclast differentiation associated genes responsible for bone resorption. Furthermore, to reduce isoflavone amount for the administration to OVX mice, a combination of isoflavone and indigestible sugars should be considered. The effective lowest dose was confirmed whether it might be about 0.05% as the aglycone daidzein.
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