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Elucidation on the fumonisin synthesis pathway of Aspergillus niger strains found in Japan

Research Project

Project/Area Number 24580208
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Food science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

NAKAGAWA Hiroyuki  独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品安全研究領域, 主任研究員 (30308192)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Kiminori  千葉大学, 真菌医学研究センター, 助教 (40345004)
YOKOYAMA Koji  千葉大学, 真菌医学研究センター, 准教授 (80092112)
Co-Investigator(Renkei-kenkyūsha) HASHIMOTO Ruiko  千葉県衛生研究所, 細菌研究室, 主任上席研究員 (70462760)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsAspergillus niger / fumonisin / mycotoxin / フモニシン / カビ毒 / 黒麹菌 / 生合成 / 国際情報交換
Outline of Final Research Achievements

Aspergillus niger strain obtained from the Medical Mycology Research Center of Chiba University was cultured, and its production of a mycotoxin, fumonisin B2 was confrimed by LC-MS/MS. The existence of fum8 gene, a part of fumonisin synthesis cluster gene was confirmed in this strain by PCR and Southern blot analysis. Therefore, fum8 disruptants and corresponding re-introduced transformant strains were prepared. The results indicated that fum8 is closely related with the ability of fumonisin B2 production of the Aspergillus niger. Other intermediate products as well as fumonisin isomers were detected, and confirmed as fumonisin B4, C2, and C4, respectively. Among other Aspergillus niger strains in the Medical Mycology Research Center, 12 strains were tested for the existence of fum8 gene, and 5 of them were found to be harboring the fum8 gene. These 5 strains produced fumonisin B2,indicating that fum8 is a useful marker gene for checking the fumonisin production of Aspergillus niger.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] The α-oxoamine synthase gene fum8 is involved in fumonisin B2 biosynthesis in Aspergillus niger2015

    • Author(s)
      Shimizu K, Nakagawa H, Hashimoto R, Hagiwara D, Onji Y, Asano K, Kawamoto S, Takahashi H, Yokoyama K
    • Journal Title

      Mycoscience

      Volume: 56 Issue: 3 Pages: 301-308

    • DOI

      10.1016/j.myc.2014.09.001

    • NAID

      40020464376

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Aspergillus nigerによるフモニシン異性体の産生とクラスター遺伝子の関与2014

    • Author(s)
      中川博之 清水公徳 松尾洋輔 橋本ルイコ 高橋治男 横山耕治
    • Organizer
      第108回日本食品衛生学会学術講演会
    • Place of Presentation
      金沢歌劇座(石川県金沢市)
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Aspergillus nigerのフモニシンクラスター遺伝子の機能解析2013

    • Author(s)
      清水公徳,中川博之,橋本ルイコ,陰地義樹,浅野勝佳,川本進,髙橋治男,横山耕治
    • Organizer
      日本菌学会第57回大会
    • Place of Presentation
      東京農業大学(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] Aspergillus nigerのフモニシン産生とクラスター遺伝子の関与

    • Author(s)
      清水公徳、中川博之、橋本ルイコ、陰地義樹、浅野勝佳、川本進、高橋治男、横山耕治
    • Organizer
      日本マイコトキシン学会第72回学術講演会
    • Place of Presentation
      東京家政大学板橋キャンパス(東京)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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