| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Outline of Final Research Achievements |
One of cellulose synthesizing protein complex, CesD from Asaia bogorensis was expressed in E.coli and purified. Then the structure of CesD has been determined by X-ray diffraction after crystallization. It is suggested CesD is composed of octamer and forms a specific pore which related to the extrusion of cellulose fibers. The pore size seems to be different from that from Gluconacetobacter xylinus, so that may be a one of the reason for less mass production of cellulose. The localization of CesD was analyzed using antibody under microscope and is suggested to exist near outer cell membrane. In addition, CesD was expressed constantly regardless of cellular growth stage.
|
