Mechanism on the extrusion of nanofiber from protein complex on cell membrane of acetic acid bacterium.
| Project/Area Number |
24580241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Masahiro 信州大学, 学術研究院工学系, 助教 (60432168)
OKUDA Kazuo 高知大学, 教育研究部, 教授 (40152417)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | セルロース合成酵素 / Asaia bogorensis / タンパク質複合体 / 繊維排出機構 / CesDタンパク質 / 立体構造 / セルロース合成 / 酢酸菌 / CesDの構造 / CesDの局在 / バクテリアセルロース |
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| Outline of Final Research Achievements |
One of cellulose synthesizing protein complex, CesD from Asaia bogorensis was expressed in E.coli and purified. Then the structure of CesD has been determined by X-ray diffraction after crystallization. It is suggested CesD is composed of octamer and forms a specific pore which related to the extrusion of cellulose fibers. The pore size seems to be different from that from Gluconacetobacter xylinus, so that may be a one of the reason for less mass production of cellulose. The localization of CesD was analyzed using antibody under microscope and is suggested to exist near outer cell membrane. In addition, CesD was expressed constantly regardless of cellular growth stage.
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Report
(4 results)
Research Products
(5 results)
