Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Outline of Final Research Achievements |
This research project aimed to clarify the mechanisms for intracellular osmoregulation, and to investigate its applicability to the fish industry. To reveal the molecular basis of amino acid transport in the teleosts, expression patterns of 93 amino acid transporter genes, identified in this project, were determined. According to rearing experiments, osmoregulatory intracellular amino acid increase was evoked by hyperosmotic stress above a certain level and this response was likely conserved among teleost species. Several analyses strongly suggest that the amino acids related to intracellular osmoregulation were derived by decomposition of proteins, and uptake of specific amino acids from extra- to intra-cellular fluids was stimulated in response to hyperosmotic challenge. Major osmoregulatory amino acids in the muscle of teleosts can show sweet taste, indicating that exposure to proper hyperosmotic conditions can be applicable to taste improvement of cultured fish.
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