Physiological study on amino acid-based intracellular osmoregulation in the teleosts and its application for aquaculture industries.
Project/Area Number |
24580260
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General fisheries
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Research Institution | The University of Tokyo |
Principal Investigator |
WATANABE Soichi 東京大学, 農学生命科学研究科, 助教 (20507884)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 浸透圧調節 / 真骨魚類 / 遊離アミノ酸 / 細胞内アミノ酸調節 / 栄養代謝 / アミノ酸輸送体 |
Outline of Final Research Achievements |
This research project aimed to clarify the mechanisms for intracellular osmoregulation, and to investigate its applicability to the fish industry. To reveal the molecular basis of amino acid transport in the teleosts, expression patterns of 93 amino acid transporter genes, identified in this project, were determined. According to rearing experiments, osmoregulatory intracellular amino acid increase was evoked by hyperosmotic stress above a certain level and this response was likely conserved among teleost species. Several analyses strongly suggest that the amino acids related to intracellular osmoregulation were derived by decomposition of proteins, and uptake of specific amino acids from extra- to intra-cellular fluids was stimulated in response to hyperosmotic challenge. Major osmoregulatory amino acids in the muscle of teleosts can show sweet taste, indicating that exposure to proper hyperosmotic conditions can be applicable to taste improvement of cultured fish.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Effects of salinity and prolactin on gene transcript levels of ion transporters, ion pumps and prolactin receptors in Mozambique tilapia intestine2014
Author(s)
Seale AP, Stagg JJ, Yamaguchi Y, Breves JP, Soma S, Watanabe S, Kaneko T, Cnaani A, Harpaz S, Lerner DT, Grau EG
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Journal Title
General and Comparative Endocrinology
Volume: 206
Pages: 146-154
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular characterization and gene expression of Na+-K+-2Cl- cotransporter2 (NKCC2) in the gastrointestinal tract of Olive flounder (Paralichythys olivaceus) during the four days after infection with Streptococcus parauberis2013
Author(s)
Kim, Y. K., Watanabe, S., Park, S. I., Jeong, J. B., Kaneko, T., Park, M. A., and Yeo, I. K.
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Journal Title
Mar. Freshw. Behav. Physiol.
Volume: 46
Issue: 3
Pages: 145-157
DOI
Related Report
Peer Reviewed
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[Journal Article] Intestinal expression of peptide transporter 1 (PEPT1) at different life stages of Japanese eel, Anguilla japonica.2013
Author(s)
Ahn, H., Yamada, Y., Okamura, A., Tsukamoto, K., Kaneko, T. and Watanabe, S.
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Journal Title
Comparative Biochemistry and Physiology - Part B
Volume: 166
Issue: 2
Pages: 157-164
DOI
Related Report
Peer Reviewed
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[Journal Article] Differential regulation of TRPV4 mRNA levels by acclimation salinity and extracellular osmolality in euryhaline tilapia2012
Author(s)
Seale, A. P., Watanabe, S., Breves, J.P., Lerner, D. T., Kaneko, T., and Grau, E.G.
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Journal Title
Gen. Comp. Endocrinol.
Volume: 178
Issue: 1
Pages: 123-130
DOI
Related Report
Peer Reviewed
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