Analysis of the virulence related genes on the fish pathogenic bacteria residing in the rotifer intestine
| Project/Area Number |
24580270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Nagasaki University |
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Principal Investigator |
SUGA Koushirou 長崎大学, 水産・環境科学総合研究科(水産), 准教授 (60569185)
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| Co-Investigator(Renkei-kenkyūsha) |
KANAI Kinya 長崎大学, 水産・環境科学総合研究科(水産), 教授 (40145222)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 魚病細菌 / 動物プランクトン / 病原性因子 / 魚病 / シオミズツボワムシ |
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| Outline of Final Research Achievements |
To clarify the pathogenic mechanism caused by oral administration of fish pathogenic bacteria via the rotifer Brachionus plicatilis in larval fish, that the present study investigated the relationship between the bacteria and B. plicatilis. The bacteria strains, Aeromonas hydrophila, Edwardsiella tarda, Photobacterium damuselae subsp. piscicida, Pseudomonas plecoglossicida, Vibrio anguillarum and Vibrio ichthyoenteri, used at this study were selected by Pulsed-field Gel Electrophoresis genotyping method. The results from the addition of the bacteria into the culture of B. plicatilis showed that 1.0E+4 cells / ml of the bacteria did not affect their life span, fecundity, and hatching rate of amictic eggs, and an individual rotifer contained 3.67E+2 CFU of bacteria in their intestine.
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Report
(4 results)
Research Products
(12 results)
