| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Comparative genome analysis of fish pathogenic Lancefield group C Streptococcus dysgalactiae (GCSD) was performed to examine the virulent genes. The analysis revealed the serum opacity factor (SOF) gene related to the virulence of other streptococci. Three types of variations in SOF genes were observed in the sequence of the sof-FD gene in GCSD with different SOF-FD activities. To develop asero-diagnostic method for GCSD infection in fish, we attempted to identify a surface immunogenic protein (Sd-Sip) induces an antibody against GCSD. Expression of their recombinant Sd-Sip (rSd-Sip) was confirmed by immunoblot analysis, that is, its reactivity to GCSD-infected sera. An tibody detection ELISA using rSd-Sip and their usefulness for diagnosis of GCSD infection were developed. To develop a rapid diagnosis method for GCSD infection, latex beads were coated with rSd-Sip.The beads were agglutinated easily with GCSD-infected sera collected from farmed fish.
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