| Project/Area Number |
24580273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Masahiro 宮崎大学, 農学部, 教授 (20178536)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | クルマエビ / ワクチン / 生体防御因子 / サイトカイン / ＷＳＳＶ / ＰＲＤＶ / ビブリオ / 生体防御 / 遺伝子 / 生体防御遺伝子 / サイトカイン関連遺伝子 / WSSV / PRDV / 抗体用因子 / 受容体 |
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| Outline of Final Research Achievements |
After vaccination with DNA vaccine and recombinant vaccine against VP-28 protein of white spot syndrome virus, we found that the anti-bacterial peptide genes, eg. crustine, lysozyme and penaeidine genes, can be used for bio-indicators for “quasi-immunity” in kuruma shrimp (Marsupenaeus japonicus). Cytokines are known to play important roles in the mechanism for vaccine efficiency in mammals. However, little is known about them in shrimp. We found over 20 cytokine genes in kuruma shrimp. The group IL-17 and related receptor genes were found. During WSSV infection, Dicer 2 and Argonaute 2 genes showed high expression in the early stage of the infection (from 12 hours post infection). This indicates the degradation of the foreign genes by these factors is a defense system in shrimp against virus. However, during the later stage, the SOCS gene was activated and suppressed the JAK/STAT pathway, another anti-virus defense system, resulting in massive growth of virus.
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