Exploring the bio-indicators to evaluate the vaccination efficacy for shrimp "quasi-immunity".
| Project/Area Number |
24580273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Masahiro 宮崎大学, 農学部, 教授 (20178536)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | クルマエビ / ワクチン / 生体防御因子 / サイトカイン / ＷＳＳＶ / ＰＲＤＶ / ビブリオ / 生体防御 / 遺伝子 / 生体防御遺伝子 / サイトカイン関連遺伝子 / WSSV / PRDV / 抗体用因子 / 受容体 |
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| Outline of Final Research Achievements |
After vaccination with DNA vaccine and recombinant vaccine against VP-28 protein of white spot syndrome virus, we found that the anti-bacterial peptide genes, eg. crustine, lysozyme and penaeidine genes, can be used for bio-indicators for “quasi-immunity” in kuruma shrimp (Marsupenaeus japonicus). Cytokines are known to play important roles in the mechanism for vaccine efficiency in mammals. However, little is known about them in shrimp. We found over 20 cytokine genes in kuruma shrimp. The group IL-17 and related receptor genes were found. During WSSV infection, Dicer 2 and Argonaute 2 genes showed high expression in the early stage of the infection (from 12 hours post infection). This indicates the degradation of the foreign genes by these factors is a defense system in shrimp against virus. However, during the later stage, the SOCS gene was activated and suppressed the JAK/STAT pathway, another anti-virus defense system, resulting in massive growth of virus.
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Expression of the serum opacity factor gene and the variation in its upstream region in <i>Streptococcus</i> <i>dysgalactiae</i> isolates from fish2012
Author(s)
Nishiki, I., Minami, T., Chen, S.-C., Itami, T., Yoshida, T.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 58
Issue: 6
Pages: 457-463
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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[Presentation] Molecular cloning, expression analysis and knockdown of nadph oxidase gene, MjNOX, in kuruma shrimp Marsupenaeus japonicus2012
Author(s)
M. Inada, R. Sudhakaran, K. Kihara, J. Nishi, M. Yoshimine, T. Mekata, T. Kono, M. Sakai, T. Yoshida, T. Itami
Organizer
AQUA2012 Global Aquaculture – Securing our Future
Place of Presentation
Praha, Czech
Related Report
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[Presentation] Gene knock down and mRNA variants of Astakine, ancient cytokine, in Kuruma Shrimp Marsupenaeus japonicus2012
Author(s)
M. Inada, K. Kihara, A. Shiki, T. Kono, T. Yoshida, M. Sakai, T. Mekata, R. Sudhakaran, T. Itami
Organizer
The 12th Congress of the International Society of Developmental and Comparative Immunology
Place of Presentation
Fukuoka, Japan
Related Report
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[Presentation] Interaction between WSSV infection and Apoptosis-, JAK/STAT-signaling- pathway- and RNAi-related Genes in Kuruma Shrimp, Marsupenaeus japonicus2012
Author(s)
M. Inada, J. Nishi, M. Yoshimine, K. Kihara, A. Shiki, T. Kono, T. Yoshida, M. Sakai, T. Mekata, R. Sudhakaran, S. Okugawa, T. Itami
Organizer
The 12th Congress of the International Society of Developmental and Comparative Immunology
Place of Presentation
Fukuoka, Japan
Related Report
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