Characterization of teleost helper T cell subsets

• Project/Area Number: 24580284
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nihon University
• Principal Investigator: MORITOMO Tadaaki 日本大学, 生物資源科学部, 教授 (20239677)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 魚類 / ヘルパーT細胞 / クローン化T細胞株 / T細胞クローン株 / Th亜集団 / ペルパーT細胞亜集団

Outline of Final Research Achievements

To identify teleost helper T cell (Th cell), we tried to establish clonal T cell lines from carp (Cyprinus carpio). Kidney leukocytes were obtained from kidney, and cultured with feeder cells (fibroblasts cell layer). In this culture condition, carp Th cells selectively proliferated, and single Th cells were picked and seeded into a well of 96 well plates. Untill now, 10 Th clones have been obtained. We found some cell lines predominantly produced Th1 cytokine (IFNg), and other cell lines predominantly produced Th2 cytokines (IL-4/13A and B).

Research Products

• [Journal Article] Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species. (2014)
  • Author(s): Takizawa, F., Araki, K., Ohtani, M., Toda, H., Saito, Y., Lampe, V.S., Dijkstra, J.M., Ototake, M., Moritomo, T., and Nakanishi, T.
  • Journal Title: Fish & shellfish immunology Volume: 36 Issue: 1 Pages: 215-222
  • DOI: 10.1016/j.fsi.2013.11.004
  • Peer Reviewed / Open Access
• [Journal Article] Peculiar monomeric interferon gammas, IFNγrel 1 and IFNγrel 2, in ginb una crucian carp. (2014)
  • Author(s): Shibasaki Y., Yabu T., Araki K., Manol N., Shiba H., Moritomo T, Nakanishi T.
  • Journal Title: FEBS Journal Volume: 281 Issue: 4 Pages: 1046-1056
  • DOI: 10.1111/febs.12666
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a novel fish granzyme involved in cell-mediated immunity. (2014)
  • Author(s): Matsuura, Y., Yabu, T., Shiba, H., Moritomo, T., and Nakanishi, T.
  • Journal Title: Developmental & Comparative Immunology Volume: 46 Issue: 2 Pages: 499-507
  • DOI: 10.1016/j.dci.2014.06.006
  • Peer Reviewed / Open Access
• [Journal Article] Nakanishi Expression profiles of interferon gamma genes in response to immune-stimulants and alloantigen in ginbuna crucian carp Carassius auratus langsdorfii (2013)
  • Author(s): Araki, K., F. Takizawa, M. Yamasaki, M. Esumi, T. Moritomo, M. Ototake, A. Yamamoto, T
  • Journal Title: Fisheries Science Volume: 79巻 Issue: 2 Pages: 213-220
  • DOI: 10.1007/s12562-012-0590-5
  • Peer Reviewed
• [Journal Article] Clonal growth of carp (Cyprinus carpio)T cells in vitro : Long-term proliferation of Th2-like cells (2012)
  • Author(s): Yamaguchi, T., Katakura. E., Someya, K., Dijkstra, J.M., Moritomo, T., Nakanishi, T.
  • Journal Title: Fish and shellfish immunology Volume: 34 Issue: 2 Pages: 433-442
  • DOI: 10.1016/j.fsi.2012.11.005
  • Peer Reviewed
• [Presentation] 魚類造血機構の解明
  • Author(s): 森友忠昭
  • Organizer: 日本魚病学会
  • Place of Presentation: 函館国際ホテル
  • Invited
• [Presentation] コイIL-2関連サイトカインの遺伝子クローニングとT細胞培養系における発現解析
  • Author(s): 銘苅 裕二，石原 裕美，染谷 和江，山口 卓哉，藪 健史，森友 忠昭，中西 照幸
  • Organizer: 日本比較免疫学会 第２５回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] GVHD誘導に伴うドナーTリンパ球における免疫関連遺伝子の発現解析
  • Author(s): 岡本修太,柴崎康宏,松浦雄太,薮健史,森友忠昭,中西照幸
  • Organizer: 日本比較免疫学会 第２５回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] コイの各臓器におけるγδT細胞の多様性について
  • Author(s): 宮前 二朗、四反田 聡，山口 卓哉，森友 忠昭，中西 照幸
  • Organizer: 日本比較免疫学会 第２５回学術集会
  • Place of Presentation: 岡山理科大
• [Presentation] コイのヘルパーＴ細胞亜集団
  • Author(s): 染谷 和江・山口 卓哉・藪 健史・森友 忠昭・中西 照幸
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 下関水産大学校
• [Presentation] Clonal culture of carp Th2-like cells whose immune status is hallmarked by IL-4/13B gene expression
  • Author(s): T. Yamaguchi, F. Katakura, J.M. Dijkstra, T. Moritomo, and T. Nakanishi
  • Organizer: 12th International society of developmental and comparative immunology
  • Place of Presentation: Hilton Fukuoka Sea Hawk Hotel
• [Presentation] Carp thrombopoietin in combination with kit ligand a induces the formation of colonies derived from unipotent progenitors for thrombocytes and bipotent progenitors for thrombocytes and erythrocytes
  • Author(s): Y. Sugie, F. Katakura, T. Yamaguchi1, T. Kato, T. Yabu, T. Moritomo and T. Nakanishi
  • Organizer: 12th International society of developmental and comparative immunology
  • Place of Presentation: Hilton Fukuoka Sea Hawk Hotel
• [Remarks] http://hp.brs.nihon-u.ac.jp/~vetfish/start.htm
• [Remarks] 研究概要
  • URL: http://hp.brs.nihon-u.ac.jp/~vetfish/start.htm