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Characterization of teleost helper T cell subsets

Research Project

Project/Area Number 24580284
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General fisheries
Research InstitutionNihon University

Principal Investigator

MORITOMO Tadaaki  日本大学, 生物資源科学部, 教授 (20239677)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords魚類 / ヘルパーT細胞 / クローン化T細胞株 / T細胞クローン株 / Th亜集団 / ペルパーT細胞亜集団
Outline of Final Research Achievements

To identify teleost helper T cell (Th cell), we tried to establish clonal T cell lines from carp (Cyprinus carpio). Kidney leukocytes were obtained from kidney, and cultured with feeder cells (fibroblasts cell layer). In this culture condition, carp Th cells selectively proliferated, and single Th cells were picked and seeded into a well of 96 well plates. Untill now, 10 Th clones have been obtained. We found some cell lines predominantly produced Th1 cytokine (IFNg), and other cell lines predominantly produced Th2 cytokines (IL-4/13A and B).

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (7 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Transcription analysis of two Eomesodermin genes in lymphocyte subsets of two teleost species.2014

    • Author(s)
      Takizawa, F., Araki, K., Ohtani, M., Toda, H., Saito, Y., Lampe, V.S., Dijkstra, J.M., Ototake, M., Moritomo, T., and Nakanishi, T.
    • Journal Title

      Fish & shellfish immunology

      Volume: 36 Issue: 1 Pages: 215-222

    • DOI

      10.1016/j.fsi.2013.11.004

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Peculiar monomeric interferon gammas, IFNγrel 1 and IFNγrel 2, in ginb una crucian carp.2014

    • Author(s)
      Shibasaki Y., Yabu T., Araki K., Manol N., Shiba H., Moritomo T, Nakanishi T.
    • Journal Title

      FEBS Journal

      Volume: 281 Issue: 4 Pages: 1046-1056

    • DOI

      10.1111/febs.12666

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a novel fish granzyme involved in cell-mediated immunity.2014

    • Author(s)
      Matsuura, Y., Yabu, T., Shiba, H., Moritomo, T., and Nakanishi, T.
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 46 Issue: 2 Pages: 499-507

    • DOI

      10.1016/j.dci.2014.06.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nakanishi Expression profiles of interferon gamma genes in response to immune-stimulants and alloantigen in ginbuna crucian carp Carassius auratus langsdorfii2013

    • Author(s)
      Araki, K., F. Takizawa, M. Yamasaki, M. Esumi, T. Moritomo, M. Ototake, A. Yamamoto, T
    • Journal Title

      Fisheries Science

      Volume: 79巻 Issue: 2 Pages: 213-220

    • DOI

      10.1007/s12562-012-0590-5

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clonal growth of carp (Cyprinus carpio)T cells in vitro : Long-term proliferation of Th2-like cells2012

    • Author(s)
      Yamaguchi, T., Katakura. E., Someya, K., Dijkstra, J.M., Moritomo, T., Nakanishi, T.
    • Journal Title

      Fish and shellfish immunology

      Volume: 34 Issue: 2 Pages: 433-442

    • DOI

      10.1016/j.fsi.2012.11.005

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 魚類造血機構の解明

    • Author(s)
      森友忠昭
    • Organizer
      日本魚病学会
    • Place of Presentation
      函館国際ホテル
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] コイIL-2関連サイトカインの遺伝子クローニングとT細胞培養系における発現解析

    • Author(s)
      銘苅 裕二,石原 裕美,染谷 和江,山口 卓哉,藪 健史,森友 忠昭,中西 照幸
    • Organizer
      日本比較免疫学会 第25回学術集会
    • Place of Presentation
      岡山理科大
    • Related Report
      2013 Research-status Report
  • [Presentation] GVHD誘導に伴うドナーTリンパ球における免疫関連遺伝子の発現解析

    • Author(s)
      岡本修太,柴崎康宏,松浦雄太,薮健史,森友忠昭,中西照幸
    • Organizer
      日本比較免疫学会 第25回学術集会
    • Place of Presentation
      岡山理科大
    • Related Report
      2013 Research-status Report
  • [Presentation] コイの各臓器におけるγδT細胞の多様性について

    • Author(s)
      宮前 二朗、四反田 聡,山口 卓哉,森友 忠昭,中西 照幸
    • Organizer
      日本比較免疫学会 第25回学術集会
    • Place of Presentation
      岡山理科大
    • Related Report
      2013 Research-status Report
  • [Presentation] コイのヘルパーT細胞亜集団

    • Author(s)
      染谷 和江・山口 卓哉・藪 健史・森友 忠昭・中西 照幸
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      下関水産大学校
    • Related Report
      2012 Research-status Report
  • [Presentation] Clonal culture of carp Th2-like cells whose immune status is hallmarked by IL-4/13B gene expression

    • Author(s)
      T. Yamaguchi, F. Katakura, J.M. Dijkstra, T. Moritomo, and T. Nakanishi
    • Organizer
      12th International society of developmental and comparative immunology
    • Place of Presentation
      Hilton Fukuoka Sea Hawk Hotel
    • Related Report
      2012 Research-status Report
  • [Presentation] Carp thrombopoietin in combination with kit ligand a induces the formation of colonies derived from unipotent progenitors for thrombocytes and bipotent progenitors for thrombocytes and erythrocytes

    • Author(s)
      Y. Sugie, F. Katakura, T. Yamaguchi1, T. Kato, T. Yabu, T. Moritomo and T. Nakanishi
    • Organizer
      12th International society of developmental and comparative immunology
    • Place of Presentation
      Hilton Fukuoka Sea Hawk Hotel
    • Related Report
      2012 Research-status Report
  • [Remarks] http://hp.brs.nihon-u.ac.jp/~vetfish/start.htm

    • Related Report
      2014 Annual Research Report
  • [Remarks] 研究概要

    • URL

      http://hp.brs.nihon-u.ac.jp/~vetfish/start.htm

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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