Development of techniques for improvement of disease resistance using transcript levels of gastrointestinal hormones as an indicator for health examination in chum salmon fry

• Project/Area Number: 24580287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido Research Organization
• Principal Investigator: MIZUNO Shinya 地方独立行政法人北海道立総合研究機構, その他部局等, 研究員 (70442655)
• Co-Investigator(Renkei-kenkyūsha): HATAKEYAMA Makoto 北海道立総合研究機構さけます, 内水面水産試験場 (80442656)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: サケ / コレシストキニン / 細菌性鰓病 / 亜麻仁油 / 脳 / シロザケ / グレリン

Outline of Final Research Achievements

This study showed that transcript level of brain cholecystokinin L-type was an indicator to predict appearance of bacterial gill disease in chum salmon fry. The cholecystokinin analysis revealed that there were no signs for crisis of bacterial gill disease in the fry of 10 hatcheries which were selected at random in Hokkaido. It was suggested that outbreak of bacterial gill disease was suppressed by daily supply of formula feed supplemented with linseed oil for a week and over.

Research Products

• [Journal Article] Effect of soaking artificially fertilized eggs in a green tea extract solution on the elimination of egg adhesiveness, hatching rate and larval quality in Japanese smelt Hypomesus nipponensis (2013)
  • Author(s): Shinya Mizuno, Makoto Hatakeyama, Tetsuo Teranishi and Nobuhisa Koide
  • Journal Title: Aquaculture Science Volume: 61 Pages: 153-162
  • NAID: 130005062505
  • Peer Reviewed
• [Journal Article] Changes in activity and transcript level of liver and gill metabolic enzymes during smoltification in masu salmon Oncorhynchus masou (2012)
  • Author(s): Shinya Mizuno, Hirokazu Urabe, Tomoya Aoyama, Hajime Omori, Anai Iijima, Kiyoshi Kasugai, Mitsuru Torao, Naoyuki Misaka, Nobuhisa Koide and Hiroshi Ueda
  • Journal Title: Aquaculture Volume: 362-363 Pages: 109-120
  • Peer Reviewed
• [Journal Article] Studies on improvement of seed production techniques in salmonids and osmerids (2012)
  • Author(s): Shinya Mizuno
  • Journal Title: Aqua-Bioscience Monographs Volume: 5 Pages: 103-143
  • Peer Reviewed
• [Presentation] 細菌性鰓病の発症に伴うシロザケ稚魚の消化管ホルモン遺伝子の発現変化 (2015)
  • Author(s): 水野伸也・畑山誠・鈴木邦夫・上田宏
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-28
• [Presentation] シロザケコレシストキニンおよびグレリンのcDNAクローニングと遺伝子発現量の解析 (2013)
  • Author(s): 水野伸也・畑山誠・小出展久・上田宏
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
• [Presentation] Effects of diets supplemented with herb essential oils on infection of parasitic trichodinid ciliates and physiology in chum salmon Oncorhynchus keta fry (2012)
  • Author(s): Shinya Mizuno, Makoto Hatakeyama, Nobuhisa Koide and Shigehiko Urawa
  • Organizer: 第10回日韓・韓日水産増殖シンポジウム
  • Place of Presentation: 長崎大学
• [Presentation] Development and Improvement of Seed Production Techniques in Osmerid Fishes
  • Author(s): Shinya Mizuno, Makoto Hatakeyama, Shuichi Mano, Tetsuo Teranishi, Noriko Sasaki, Yoshitaka Sasaki, Hirofumi Hayano and Nobuhisa Koide
  • Organizer: 2nd World Congress of Mariculture and Fisheries
  • Place of Presentation: Hangzhou Blossom Water Museum Hotel, Hangzhou, China
  • Invited
• [Presentation] 北海道の河川に生息する野生魚の原虫寄生状況
  • Author(s): 水野伸也・畑山誠・宮本真人・實吉隼人・春日井潔・小山達也・竹内勝巳・小出展久・浦和茂彦
  • Organizer: 平成25日本水産増殖学会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] シロザケ稚魚のコレシストキニンおよびグレリン遺伝子の発現量に与える成長および飼育環境の影響
  • Author(s): 水野伸也・畑山誠・小出展久・上田宏
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道大学函館キャンパス
• [Book] Salmon Biology, Ecological Impacts and Economic Importance (Woo PTK & Noakes DJ. eds.) Seed quality in hatchery-reared chum salmon fry: Methods of evaluation and improvement (2014)
  • Author(s): Shinya Mizuno
  • Publisher: NOVA Science Publisher
• [Book] Salmon: Biology, Ecological Impacts and Economical Importance (2014)
  • Author(s): Shinya Mizuno
  • Total Pages: 12
  • Publisher: Nova Science