| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Most of the musculature in echinoderms are smooth muscle and the contraction of which is believed to be regulated by the phosphorylation of myosin light chain depending on intracellular Ca2+ concentration as in the case of other smooth muscles in higher animals. In this study, troponin which is known as an actin-linked regulatory protein of striated muscles was revealed to be present in the various smooth muscles of sea urchin. The subunits of sea urchin troponin were obtained by the expression system constructed in Escherichia coli and were confirmed to be functional in vitro, even though they are lacking some regions which are known to be essential for the Ca2+-dependent activation of vertebrate striated muscle contraction. Therefore, the sea urchin troponin should activate the contraction by the acting mechanisms different from those of vertebrate troponin.
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