development of fish roe allergen detection system using IgE epitope structure information

• Project/Area Number: 24580294
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Hokkaido University
• Principal Investigator: SAEKI Hiroki 北海道大学, 水産科学研究科(研究院), 教授 (90250505)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Yutaka 北海道大学, 大学院水産科学研究院, 技術専門職員 (00374629) ; HIRAMATSU Naoshi 北海道大学, 大学院水産科学研究院, 准教授 (10443920) ; HARA Akihiko 北海道大学, 名誉教授 (40091483)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 食物アレルギー / 魚卵 / β’-component / IgEエピトープ / ペプチド抗体 / シロザケ / カレイ / カペリン / 抗エピトープ抗体 / アサバカレイ / アレルゲン / β'-コンポーネント / IgE結合部位 / いくら / β’-コンポーネント / 抗原交差性 / 抗ペプチド抗体

Outline of Final Research Achievements

To investigate ELISA system for detecting fish roe common major allergen, β’-component, a series of antibodies were prepared utilizing sequential IgE-epitopes and their analog sequences. Some of these antibodies reacted to only intact β’-component, and some of the others reacted to β’-component that was collapsed its tertiary structure by enzymatic degradation or SDS-treatment. Although peptide-antibody with the same reactivity to all β’-component was not obtained, the application of the series of antibody could contribute to development of qualitative ELISA system for detection of wide range of fish roe allergen and to detection of structural change around each IgE-epitope. The results of this research suggest that the use of peptide-antibodies that recognize IgE epitopes could be an effective tool for studying the structural changes in the food allergens.

Research Products

• [Journal Article] Molecular and Immunological Characterization of β’-component (Onc k 5), a major IgE-binding protein in chum salmon roe (2013)
  • Author(s): Yutaka Shimizu, Hideki Kishimura, Gaku Kanno, Atsushi Nakamura, Reiko Adachi, Hiroshi Akiyama, Kazuhiko Watanabe, Akihiko Hara,Motohiro Ebisawa, and Hiroki Saeki
  • Journal Title: International Immunology Volume: 26 Issue: 3 Pages: 139-147
  • DOI: 10.1093/intimm/dxt051
  • Peer Reviewed
• [Presentation] 魚卵アレルゲンのIgE反応性変化を調べるツールとしての抗ペプチド抗体の有用性 (2014)
  • Author(s): 清水裕、平松尚志、佐伯宏樹
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 北海道酪農学園大学（北海道江別市）
  • Year and Date: 2014-05-31
• [Presentation] ペプチド抗体を用いた魚卵アレルギーの魚種間交差性に関する研究
  • Author(s): 清水 裕，岸村栄毅、平松尚志、原彰彦、佐伯宏樹
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学（東京都港区）
• [Presentation] Major allergen in gray mullet roe and its IgE cross-reactovity to chum salmon roe
  • Author(s): Y. Shimizu, T. Lee, H. Kishimura, A. Hara, K. Watanabe, H. Saeki
  • Organizer: International Society for Nutraceuticals and Functional Foods
  • Place of Presentation: Howard Civil Service International House（Taipei, Taiwan）
• [Presentation] ペプチド抗体を用いた魚卵アレルギーの魚種間交差性に関する検討
  • Author(s): 清水 裕、岸村栄毅、平松尚志、原 彰彦、佐伯宏樹
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都）
• [Remarks] Allergen Nomencleature Database
  • URL: http://www.allergen.org/viewallergen.php?aid=764