Endocrine regulation of triacylglycerol-transporting molecules involved in species-specific lipid accumulation in fish
| Project/Area Number |
24580297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANEKO Gen 東京大学, 農学生命科学研究科, 助教 (30466809)
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| Co-Investigator(Renkei-kenkyūsha) |
KONDO Hidehiro 東京海洋大学, 海洋科学技術研究科, 准教授 (20314635)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂質 / リパーゼ / 成長ホルモン / トラフグ / マダイ / ヒラメ / 脂質輸送 / トリアシルグリセロール / リポタンパク質 / リポタンパク質リパーゼ |
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| Outline of Final Research Achievements |
In this project, we first determined lipid distribution patterns across 10 species by oil red O staining. The mRNA levels of lipoprotein lipase (LPL) were well associated with the lipid distribution patterns, suggesting that the species-specific lipid distribution was attributed to the differences in lipid incorporation to skeletal muscle. We also characterized hormone-sensitive lipase (HSL), which is involved in lipolysis under fasting conditions, in several fish species. Subsequent ex vivo experiments revealed that the expression of LPL and HSL were regulated by growth hormone. Taken together with the species-specific distribution patterns of growth hormone receptor, we proposed a hypothesis that sensitivity to this hormone is a major determinant of lipid distribution patterns in fish.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] DNA microarray analysis on the genes differentially expressed in the liver of the pufferfish Takifugu rubripes following an intramuscular administration of tetrodotoxin.2014
Author(s)
Matsumoto T, Feroudj H, Kikuchi R, Kawana Y, Kondo H, Hirono I, Aki S, Mochizuki T, Nagashima Y, Kaneko G, Ushio H, Kodama M, Watabe S.
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Journal Title
Microarrays
Volume: 3
Issue: 4
Pages: 226-244
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] DNA microarray analysis on gene candidates possibly related to tetrodotoxin accumulation in pufferfish2014
Author(s)
Holger Feroudj, Takuya Matsumoto, Yohei Kurosu, Gen Kaneko, Hideki Ushio, Katsuaki Suzuki, Hidehiro Kondo, Ikuo Hirono, Yuji Nagashima, Seiji Akimoto, Kazushige Usui, Shigeharu Kinoshita, Shuichi Asakawa, Masaaki Kodama, Shugo Watabe
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Journal Title
Toxicon
Volume: 77
Pages: 68-72
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream2013
Author(s)
Kaneko G, Yamada T, Han Y, Hirano Y, Khieokhajonkhet A, Shirakami H, Nagasaka R, Kondo H, Hirono I, Ushio H, Watabe S
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Journal Title
Gen Comp Endocrinol
Volume: 184
Pages: 51-60
DOI
Related Report
Peer Reviewed
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[Journal Article] Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream.2013
Author(s)
Kaneko G*, Yamada T, Han Y, Hirano Y, Khieokhajonkhet A, Shirakami H, Nagasaka R, Kondo H, Hirono I, Ushio H, Watabe S.
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Journal Title
General and Comparative Endocrinology
Volume: 184
Pages: 51-60
Related Report
Peer Reviewed
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