Search of the molecular marker concerned with seafood allergic disease and the application to an allergenicity evaluation

• Project/Area Number: 24580301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: ISHIZAKI SHOICHIRO 東京海洋大学, 海洋科学技術研究科, 准教授 (40251681)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アレルギー / 食品 / アレルゲン / 魚介類 / 遺伝子発現 / 魚貝類

Outline of Final Research Achievements

Seafoods, including fish, crustaceans and mollusks, are well recognized as one of the most common causes of food allergy mediated by immunoglobulin E antibodies. However, the mechanism concerning to onset of seafood allergy is not elucidated, and the details of the gene expression in conjunction with the onset are not clarified.
Therefore, in this study, seafood allergy model mouse was constructed for the purpose of the identification of the gene cluster about onset of seafood allergy. As a result, the gene which the expression increased was specifically identified in case of the onset of seafood allergy by the cDNA subtraction method. It was revealed that a kind of cytokine called interleukin-5 specifically participated in the onset of seafood allergy.

Research Products

• [Journal Article] Paramyosin from the Disc Abalone Haliotis Discus Discus (2014)
  • Author(s): M. Suzuki, K. Shimizu, Y. Kobayashi, S. Ishizaki, K. Siomi
  • Journal Title: Journal of Food Biocemistry Volume: 38 Issue: 4 Pages: 444-451
  • DOI: 10.1111/jfbc.12072
  • Peer Reviewed / Open Access
• [Journal Article] Importance of conformation for the IgE reactivity of sarcoplasmic calcium-binding protein from the black tiger shrimp Penaeus monodon (2013)
  • Author(s): A. Morii, H. Mita, S. Ishizaki, and K. Shiomi
  • Journal Title: European Food Research and Technology Volume: 236 Issue: 1 Pages: 165-170
  • DOI: 10.1007/s00217-012-1867-8
  • Peer Reviewed
• [Journal Article] Molecular cloning and functional expression of allergenic sarcoplasmic calcium-binding proteins from Penaeus shrimps (2013)
  • Author(s): H. Mita, A. Koketsu, S. Ishizaki, and K. Shiomi
  • Journal Title: Journal of the Science of Food and Agriculture Volume: 93 Issue: 7 Pages: 1737-1742
  • DOI: 10.1002/jsfa.5961
  • Peer Reviewed
• [Journal Article] Specific Detection by the Polymerase Chain Reaction of Potentially Allergenic Salmonid Fish Residues in Processed Foods (2012)
  • Author(s): S. Ishizaki, Y. Sakai, T. Yano, S. Nakano, T. Yamada, Y. Nagashima, K. Shiomi, Y. Nakao, and H. Akiyama
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 5 Pages: 980-985
  • DOI: 10.1271/bbb.110992
  • NAID: 10030752985
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] 各種軟体動物のパラミオシンのアレルゲン性および一次構造解析 (2012)
  • Author(s): 清水景子・鈴木 翠・嶋倉邦嘉・石崎松一郎・塩見一雄
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 山口県下関市，独立行政法人水産大学校
• [Presentation] マサバパルブアルブミンの立体構造IgE結合エピトープの解析 (2012)
  • Author(s): 森井愛花・小林征洋・田中雄太・嶋倉邦嘉・石崎松一郎・塩見一雄
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 山口県下関市，独立行政法人水産大学校