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Molecular mechanisms of desiccation-tolerance in the Pyropia yezoensis chloroplast translation system

Research Project

Project/Area Number 24580303
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Fisheries chemistry
Research InstitutionNagasaki University

Principal Investigator

YAMAGUCHI Kenichi  長崎大学, 水産・環境科学総合研究科(水産), 准教授 (90363473)

Co-Investigator(Kenkyū-buntansha) ODA Tatsuya  長崎大学, 水産・環境科学総合研究科(水産), 教授 (60145307)
KUWANO Kazuyoshi  長崎大学, 水産・環境科学総合研究科(水産), 教授 (60301363)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsリボソームタンパク質 / LEA / 葉緑体 / スサビノリ / LEA様タンパク質 / 葉緑体リボソームタンパク質 / 該当なし
Outline of Final Research Achievements

Desiccation-tolerant leafy gametophyte of the red alga Pyropia yezoensis is commercially important marine crop for the major source of dried Nori (Nori sheets). We recently found that dried Nori contains abundant chloroplast ribosomes as 70S-type particles. In this study, we explored proteinaceous factors, which may confer desiccation-tolerance and heat-stability, by means of proteomic approaches. Consequently, we found novel LEA-like proteins that are likely localized in the chloroplast stroma. We also suggest that the protein composition of P. yezoensis chloroplast ribosome and its translation factors resembles that of mesophilic bacteria (e.g., E. coli).

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): protective activity of the N-terminal fragment of PYP1 against acetaminophen-induced cell death in Chang liver cells.2015

    • Author(s)
      Choi YH, Yamaguchi K, Oda T, Nam TJ.
    • Journal Title

      Int J Mol Med.

      Volume: 35 Issue: 1 Pages: 271-276

    • DOI

      10.3892/ijmm.2014.1992

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] スサビノリ葉状体タンパク質の二次元電気泳動に有効なTRIzol法と段階的可溶化によるサブプロテオーム分画2015

    • Author(s)
      山口健一、森悠江、柴田-山脇萌美、金井剛志、桑野和可、小田達也
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス(東京都港区)
    • Year and Date
      2015-03-27 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] スサビノリ葉状体の乾燥下のタンパク質発現プロファイリングと非乾燥誘導性LEA様タンパク質の同定2014

    • Author(s)
      山口健一、島祐理、河野なつみ、柴田-山脇萌美、山倉那央、金井剛志、桑野和可、小田達也
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      九州大学箱崎キャンパス(福岡県福岡市東区)
    • Year and Date
      2014-09-19 – 2014-09-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 葉緑体タンパク質合成系のプロテオミクス:緑色植物と紅藻2014

    • Author(s)
      山口健一
    • Organizer
      第37回 蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      久山レイクサイドホテル(福岡県糟屋郡久山町)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] スサビノリプロテオームの二次元電気泳動に適したサンプル調製法の検討2012

    • Author(s)
      山脇萌美、山口健一、小田達也
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      下関
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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