Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Outline of Final Research Achievements |
Membrane translocating motif (MTM) composes a signal peptide sequence of hydrophobic amino acid residues, AAVLLPVLLAAP. To develop a technology to introduce directly signal transducing molecules into zebrafish embryos, we generated a recombinant MTM-tagged p53 (MTM-p53) in E.coli and then examined the transcriptional activity of the purified MTM-p53 in vitro and in vivo. we developed in here a novel transducing technology that is able to directly introduce to zebrafish and then to functionally act MTM-p53 protein . This provides a useful approach to understand the spatiotemporal regulatory mechanism(s) of signal transducing molecules in zebrafish.
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