Development of a novel technology to introduce signal transduction molecules into zebrafish embryo using membrane-translocating motif
Project/Area Number |
24580308
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Fisheries chemistry
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Research Institution | Nihon University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MASUHIRO Yoshikazu 日本大学, 生物資源科学部, 准教授 (00336083)
YABU Takeshi 日本大学, 生物資源科学部, 研究員 (00551756)
IMAMURA Shintarou 独立行政法人水産総合研究センター, 研究員 (80510007)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | ゼブラフィッシュ / 細胞膜透過性ペプチド / シグナル分子 / キメラ蛋白質 / 形態形成 / 細胞分化 / 可視化 / 細胞膜透過性シグナル分子 / p53 / 転写能 / 機能発現 / 細胞膜透過性分子 / TIPE2 / 細胞膜透過性蛋白質 / 蛋白質発現 |
Outline of Final Research Achievements |
Membrane translocating motif (MTM) composes a signal peptide sequence of hydrophobic amino acid residues, AAVLLPVLLAAP. To develop a technology to introduce directly signal transducing molecules into zebrafish embryos, we generated a recombinant MTM-tagged p53 (MTM-p53) in E.coli and then examined the transcriptional activity of the purified MTM-p53 in vitro and in vivo. we developed in here a novel transducing technology that is able to directly introduce to zebrafish and then to functionally act MTM-p53 protein . This provides a useful approach to understand the spatiotemporal regulatory mechanism(s) of signal transducing molecules in zebrafish.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Novel Selenium-Containing Compound, Mediates Detoxification Mechanisms against Methylmercury Accumulation and Toxicity in Zebrafish Embryo.2013
Author(s)
M. Yamashita, Y. Yamashita, T. Suzuki, Y. Kani, N. Mizusawa, S. Imamura, K. Takemoto, T. Hara, Md. A. Hossain, T. Yabu, K. Touhata.
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Journal Title
Mar. Biotechnol.,
Volume: 15
Pages: 559-570
Related Report
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