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Fermented beet top feed production with control of redox potential on cellulose and hemicellulose hydrolyzing microbes

Research Project

Project/Area Number 24580384
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

TAKAHASHI JUNICHI  帯広畜産大学, その他部局等, 名誉教授 (20111198)

Co-Investigator(Kenkyū-buntansha) NISHIDA Takehiro  帯広畜産大学, 畜産学部, 准教授 (70343986)
Research Collaborator HAMAMOTO Osamu  
O'Brien Martin  
NAKAMURA Yukio  
YAMASHIRO Takaki  
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsバイオマス / リサイクル / ビートトップ / セルロース・ヘミセルロース加水分解 / 生菌剤 / 酸化還元電位制御バイオリアクター / 体細胞数 / 残留農薬 / 酪農TMR飼料 / セルロース / ヘミセルロース / 農業バイオマス / 電位制御リアクター / 繊維加水分解 / 電位制御バイオリアクター / 繊維加水分解菌 / 乳酸発酵 / TMR / 乳牛 / 印加電圧 / セルロースバイオマ / 導電性カーボンフェルト
Outline of Final Research Achievements

Recycling of beet top for the dairy fermented feed was examined using mixed microbial preparation (BIO-PKC). To secure the safety and conservation of the BIO-PKC fermented beet top oxalate and nitrate contents, coliform bacteria, mycotoxin and residual pesticides were analyzed and secured. Feed value of BIO-PKC fermented beet top as a dairy feed ingredient was demonstrated by the digestion and nitrogen balance trials using sheep and feeding trial as the total mixed ration using the milking cows in the conventional dairy farm. The possible suppression of somatic cell counts suggested a prophylactic effect of BIO-PKC on mastitis of dairy cow. To activate cellulose and hemicellulose hydrolyzing ability of BIO-PKC by the control of oxidation–reduction potential the bioreactor equipped with redox potential controller was developed. In the fermentation with voltage applied BIO-PKC the hydrolases of cellulose and hemicellulose of beet top was accelerated and eventually saccharified.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (40 results)

All 2015 2014 2013 2012 Other

All Int'l Joint Research (3 results) Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 13 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Book (12 results) Remarks (3 results)

  • [Int'l Joint Research] University of Sydney(オーストラリア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Institute of Animal Science/Nutrivet(チェコ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Unilever/同済大学環境科学工学院(中国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Effect of the activity of coprophagous insects on greenhouse gas emissions from cattle dung pats and changes in amount of nitrogen, carbon, and energy. Environmental Entomology,.2015

    • Author(s)
      Iwasa, M., Y. Mori and J. Takahashi,
    • Journal Title

      Environmental Entomology,

      Volume: 44

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hygienic treatment and energy recovery of dead animals by high solid co-digestion with vinasse under mesophilic condition: feasibility study.2015

    • Author(s)
      Dai, X., S. Chen, Y. Xue, L. D. Dai, N. L. J. Takahashi and W. Zhao,
    • Journal Title

      Journal of Hazardous Materials,

      Volume: 297 Pages: 320-328

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 地域共生型社会におけるバイオガスプラントの新たな機能について-分散型負荷平準化施設としての寄与-.2015

    • Author(s)
      浜本 修・高橋潤一.
    • Journal Title

      .日本大学生物資源科学部人文社会系研究紀要,

      Volume: 12 Pages: 1252-1262

    • NAID

      40020365567

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Li Hygienic treatment and energy recovery of dead animals by high solid co-digestion with vinasse under mesophilic condition: feasibility study.2015

    • Author(s)
      Dai, X., S.Chen, Y. Xue, J. Takahashi., N.
    • Journal Title

      Journal of Hazardous Materials

      Volume: -

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prophylactic modulation of methane and nitrous oxide emitted from ruminants livestock for sustainable animal agriculture (REVIEW).2014

    • Author(s)
      Takahashi, J.
    • Journal Title

      Journal of Animal Science and Technology

      Volume: 37 Pages: 169-174

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of the activity of coprophagous insects on greenhouse gas emissions from cattle dung pats and changes in amount of nitrogen, carbon, and energy.2014

    • Author(s)
      Iwasa, M., Y. Mori and J. Takahashi
    • Journal Title

      Environmental Entomology

      Volume: 44 Pages: 106-113

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] New insight into chemical changes of dissolved organic matter during anaerobic digestion of dewatered sewage sludge using EEM-PARAFAC and two-dimensional FTIR correlation spectroscopy.2014

    • Author(s)
      Li, X., X. Dai, J. Takahashi, N. Li, L. Dai, B. Dong
    • Journal Title

      Bioresource Technology

      Volume: 159 Pages: 412-420

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meta-analysis on Methane Mitigating Properties of Saponin-rich Sources in the Rumen: Influence of Addition Levels and Plant Sources.2014

    • Author(s)
      Jayanegara, A., E. Wina, J. Takahashi
    • Journal Title

      Asian Austral. J. Anim. Sci.

      Volume: 27 Pages: 1426-1435

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 北海道におけるビートトップの飼料化の状況について2014

    • Author(s)
      高橋潤一
    • Journal Title

      酪農ジャーナル

      Volume: 67 Pages: 22-25

    • NAID

      40020283050

    • Related Report
      2014 Research-status Report
  • [Journal Article] New insight into chemical changes of dissolved organic matter during anaerobic digestion of dewatered sewage sludge using EEM-PARAFAC and two-dimensional FTIR correlation spectroscopy2014

    • Author(s)
      Li, X., X.Dai, J. Takahashi, N. Li, L. Dai, B. Dong
    • Journal Title

      Bioresource Technology

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Meta-analysis on methane mitigating properties of saponin-rich sources in the rumen: influence of addition levels and plant sources2014

    • Author(s)
      Jayanegara, A.,E. Wina, J.Takahashi
    • Journal Title

      Asian-Australasian Journal of Animal Science

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The impact of Lactobacillus plantarum TUA1490L supernatant on in vitro rumen methanogenesis and fermentation2013

    • Author(s)
      O'Brien, M., T. Hashimoto, A. Senda, T. Nishida, J. Takahashi
    • Journal Title

      Anaerobe

      Volume: 22 Pages: 137-140

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of saponin-rich sources in relation to ruminal methane mitigation at various addition levels in vitro: an evidence from meta-analysis study2013

    • Author(s)
      Jayanegara, A., A. Sudarman, E. Wina, J. Takahashi
    • Journal Title

      Advances in Animal Biosciences (Cambridge)

      Volume: 4 Pages: 293-293

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synergistic effect of Alcaligenes faecalis and nitrate to reduce in vitro rumen methanogenesis2013

    • Author(s)
      O'Brien, M., M Shoda, T Nishida, J Takahashi
    • Journal Title

      Advances in Animal Biosciences (Cambridge)

      Volume: 4 Pages: 394-394

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Livestock and greenhouse gas emission: Bilateral impact and mitigation options for sustainable production system, Alexandria, Egypt. pp15-25.2015

    • Author(s)
      Takahashi, J.
    • Organizer
      2nd Iternational conference on the modern approaches in livestock's production systems
    • Place of Presentation
      Alexandria,Egypt
    • Year and Date
      2015-10-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ruminant feed production from cellulose biomass applied ammonia stripping from digested slurry of thermophilic biogas plant.2014

    • Author(s)
      Takahashi, J.
    • Organizer
      Proceedings of The 16th International Conference on Forage Conservation
    • Place of Presentation
      Mendel University, Brno, Czech Republic
    • Year and Date
      2014-11-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Perspective on methane production by dairy goat farm.2014

    • Author(s)
      Takahashi J.
    • Organizer
      Proceedings of the 2nd Asian-Australasian dairy Goat Conference
    • Place of Presentation
      Bogor, Indonesia
    • Year and Date
      2014-09-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Livestock and greenhouse gas emission: impact, assessment and mitigation. In: Climate Resilient Livestock Feeding Systems for Glpbal Food Security.2014

    • Author(s)
      Takahashi, J
    • Organizer
      Global Animal Nutrition Conference 2014
    • Place of Presentation
      Bangalore. India
    • Year and Date
      2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Perspective on GHG Control towards Sustainable Animal Agriculture2013

    • Author(s)
      Takahashi, J
    • Organizer
      International Conference on Animal & Dairy Sciences
    • Place of Presentation
      Las Vegas, USA
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Role of biotechnological research to support SAADC-mitigation of methane and nitrous oxide and utilization of agricultural biomass2013

    • Author(s)
      Takahashi, J., M. Shoda, J. Lee
    • Organizer
      The 4th International conference on Sustainable Animal Agriculture for Developing Countries
    • Place of Presentation
      Lanzhou, China
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] New Concepts of Biogas System for Sustainable Animal Agricultur2013

    • Author(s)
      Takahashi, J
    • Organizer
      The 15th International Conference on Forage Conservation
    • Place of Presentation
      High Tatras, Slovakia
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 発酵ビートトップ飼料創製―セルロース・ヘミセルロース加水分解菌の電位制御2013

    • Author(s)
      高橋潤一・西田武弘・中村幸夫・浜本 修
    • Organizer
      日本畜産学会第117大会
    • Place of Presentation
      新潟大学、新潟市
    • Related Report
      2013 Research-status Report
  • [Book] Livestock Production and Climate Change2015

    • Author(s)
      Takahashi, J., 2015. Greenhouse Gases and Sustainable Animal Agriculture, In: LiveP.K. Malik, R. Bhatta, J. Takahashi, R.A Kohn and C. S. Prasad .
    • Total Pages
      395
    • Publisher
      CABI, UK,
    • Related Report
      2015 Annual Research Report
  • [Book] バイオ水素とキャリア開発の最前線(植田充美監修)2015

    • Author(s)
      高橋潤一
    • Total Pages
      213
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Annual Research Report
  • [Book] アンモニアを用いた水素エネルギーシステム(小島由継監修)2015

    • Author(s)
      高橋潤一
    • Total Pages
      239
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Annual Research Report
  • [Book] Greenhouse Gases and Sustainable Animal Agriculture, In: Livestock Production and Climate Change2015

    • Author(s)
      Takahashi J.(P.K. Malik, R. Bhatta, J. Takahashi, Kohn and C. S. Prasad (eds.). )
    • Total Pages
      384
    • Publisher
      CABI
    • Related Report
      2014 Research-status Report
  • [Book] アンモニアを用いた水素エネルギーシステム 第3編アンモニア利用「第1章家畜排泄物からのアンモニア生産と燃料電池への応用」2015

    • Author(s)
      高橋潤一(小島 由継監修)
    • Publisher
      シー・エム・シー出版
    • Related Report
      2014 Research-status Report
  • [Book] バイオ水素とキャリア開発の最前線 2章 4.家畜排泄物からの水素キャリアー・アンモニア生産2014

    • Author(s)
      高橋潤一(植田充実監修)
    • Total Pages
      213
    • Publisher
      シー・エム・シー出版
    • Related Report
      2014 Research-status Report
  • [Book] Livestock Production and Climate Change2014

    • Author(s)
      Takahashi, J. (P.K. Malik, R. Bhatta, J. Takahashi, Kohn and C. S. Prasad eds.)
    • Publisher
      CABI
    • Related Report
      2013 Research-status Report
  • [Book] Lactic Acid Bacteria and Mitigation of GHG Emission from Ruminant Livestock. In: Lactic Acid Bacteria - R & D for Food, Health and Livestock Purposes2013

    • Author(s)
      Takahashi, J. (M. Kongo ed.)
    • Total Pages
      658
    • Publisher
      IN-TECH
    • Related Report
      2013 Research-status Report
  • [Book] リサイクルバイオテクノロジーの最前線2013

    • Author(s)
      高橋潤一, ( 植田充実監修)
    • Total Pages
      239
    • Publisher
      シー・エム・シー出版
    • Related Report
      2013 Research-status Report
  • [Book] リサイクルバイオテクノロジーの最前線2013

    • Author(s)
      高橋潤一
    • Total Pages
      239
    • Publisher
      シー・エム・シー出版
    • Related Report
      2012 Research-status Report
  • [Book] LACTIC ACID BACTERIA R&D FOR FOOD, HEALTH AND LIVESTOCK PURPOSES2013

    • Author(s)
      JUNICHI TAKAHASHI
    • Total Pages
      658
    • Publisher
      INTECH
    • Related Report
      2012 Research-status Report
  • [Book] バイオマス分解酵素研究の最前線―セルラーゼ・ヘミセルラーゼを中心として―2012

    • Author(s)
      高橋潤一
    • Total Pages
      311
    • Publisher
      シー・エム・シー出版
    • Related Report
      2012 Research-status Report
  • [Remarks] セルロース・ヘミセルロース加水分解菌の電位制御による発酵ビートトップの飼料創製

    • URL

      http://tech.obihiro.ac.jp/~takahashikaken/beettop/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 反芻動物における温室効果ガス抑制効果を有する天然素材インベントリー」で作成したホームページ

    • URL

      http://tech.obihiro.ac.jp/~takahashikaken/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 帯広畜産大学プロジェクト研究ホームページ

    • URL

      http://tech.obihiro.ac.jp/~takahashikaken/index.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2022-02-21  

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