Screening of avian leptin using real-time imaging assay for leptin
| Project/Area Number |
24580386
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | レプチン / 情報伝達 / バイオアッセイ / 摂食 / 比較ゲノミクス / 鳥類 / クローニング / 細胞内情報伝達 |
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| Outline of Final Research Achievements |
We identified leptin-like activity in the blood in chicken and crow using leptin specific bio assay in the present study. Furthermore, we found leptin gene in the falcon genome, and recombinant falcon leptin activates signal transduction cascade by coupling with chicken leptin receptor in vitro. the present results gave a academic significance in terminating the controversy about the existence of avian leptin.
We also investigated the role of leptin on pituitary function, and by which reveald that leptin promote the transcription of chicken growth hormone gene with Pit-1.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Discovery of the elusive leptin in birds: identification of several 'missing links' in the evolution of leptin and its receptor.2014
Author(s)
Prokop JW, Schmidt C, Gasper D, Duff RJ, Milsted A, Ohkubo T, Ball HC, Shawkey MD, Mays HL Jr, Cogburn LA, Londraville RL.
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Journal Title
PLoS One
Volume: 9
Issue: 3
Pages: e92751-e92751
DOI
Related Report
Peer Reviewed
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