Investigation into lactic acid bacteria involving in resistance to aerobic stability of total mixed ration silage

• Project/Area Number: 24580390
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Okayama University
• Principal Investigator: NISHINO Naoki 岡山大学, その他の研究科, 准教授 (50237715)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 乳酸菌 / 飼料 / サイレージ

Outline of Final Research Achievements

Evidence has shown that total mixed ration (TMR) silage can resist deterioration after silo opening. From culture-independent microbiota analysis of practical TMR silage, several difficult-to-culture lactic acid bacteria, such as Lactobacillus pontis, Lactobacillus fructivorans, were suggested to be involved. We successfully isolated L. fructivorans using liver infusion sake medium, and then confirmed its inhibitory activity against aerobic spoilage.

Research Products

• [Journal Article] Identification of bacteria in total mixed ration silage produced with and without crop silage as an ingredient. (2015)
  • Author(s): Nishino, N., Ogata, Y., Han, H. and Yamamoto, Y.
  • Journal Title: Animal Science Journal Volume: 86 Issue: 1 Pages: 45-50
  • DOI: 10.1111/asj.12234
  • Peer Reviewed
• [Journal Article] Identification of lactic acid bacteria in the rumen and feces of dairy cows fed total mixed ration silage to assess the survival of silage bacteria in the gut. (2014)
  • Author(s): Han, H., Ogata, Y., Yamamoto, Y., Nagao, S. and Nishino, N.
  • Journal Title: Journal of Dairy Science Volume: 97 Issue: 9 Pages: 5754-5762
  • DOI: 10.3168/jds.2014-7968
  • Peer Reviewed
• [Journal Article] A survey of fermentation products and bacterial communities in corn silage produced in a bunker silo in China. (2014)
  • Author(s): Wang, C., Han, H., Gu, X., Yu, Z. and Nishino, N.
  • Journal Title: Animal Science Journal Volume: 85 Issue: 1 Pages: 32-36
  • DOI: 10.1111/asj.12076
  • Peer Reviewed
• [Journal Article] A pilot examination of the fermentation products, aerobic stability and bacterial community of total mixed ration silage produced in Vietnam. (2014)
  • Author(s): Tu T.T.M., Van, N.H. and Nishino, N.
  • Journal Title: Grassland Science Volume: 60 Issue: 1 Pages: 63-68
  • DOI: 10.1111/grs.12041
  • NAID: 40020340677
  • Peer Reviewed
• [Journal Article] Bacterial communities in alfalfa and corn silages produced in large-scale stack and bunker silos in China. (2014)
  • Author(s): Wu, B., Zhang, Q., Liu, Z., Yu, Z. and Nishino, N.
  • Journal Title: Grassland Science Volume: 60 Issue: 4 Pages: 247-251
  • DOI: 10.1111/grs.12063
  • Peer Reviewed
• [Journal Article] Effects of storage temperature and ensiling period on fermentation products, aerobic stability and microbial communities of total mixed ration silage. (2013)
  • Author(s): Wang, C. and Nishino, N.
  • Journal Title: Journal of Applied Microbiology Volume: 114 Issue: 6 Pages: 1-9
  • DOI: 10.1111/jam.12200
  • Peer Reviewed
• [Presentation] Bacteria associated with ensiling fermentation and aerobic stability of total mixed ration silage.
  • Author(s): Nishino, N. and Wang, C.
  • Organizer: 16th International Silage Conference
  • Place of Presentation: Hämeenlinna, Finland
• [Presentation] ベトナムで調製した発酵TMRの貯蔵性と好気的安定性
  • Author(s): Tran Thi Minh Tu・尾形 友・Nguyen Huu Van・西野直樹
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 広島市