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Evaluation for the prevention of infection by lactic acid bacteria possessing the adhesion ability to mucin

Research Project

Project/Area Number 24580397
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionKitasato University

Principal Investigator

MUKAI Takao  北里大学, 獣医学部, 教授 (20229917)

Research Collaborator YAMAMATO Yuji  北里大学, 獣医学部, 講師 (10453507)
MATSUI Hidenori  北里大学, 生命科学研究所, 講師 (30219373)
NISHIYAMA Keita  北里大学, 獣医学系研究科
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords乳酸菌 / ビフィズス菌 / 付着 / ムチン / 翻訳伸張因子 / 感染予防 / 糖鎖 / プロバイオティクス
Outline of Final Research Achievements

The molecular mechanisms of Lactobacillus adhesion have not been fully studied. For the adhesion studies, we firstly demonstrated that the degree of mucin purification influenced adhesion properties of Lactobacillus and Bifidobacterium strains and that several strains had the ability to recognize sulfated and/or sialylated carbohydrates in mucins. Next, we examined the adhesion mechanisms of the bacteria to mucin. As a result, the present study demonstrated that EF-Tu from Lactobacillus and Bifidobacterium strains could bind to sulfated carbohydrate moieties of mucin. It was also shown that EF-Tu is probably secreted to the cell surface through a certain specific mechanism. The strain expressing Ef-Tu on the cell surfaces could partially prevent adhesion or invasion of Campylobacter or Helicobacter to the intestinal cells. Our results provide a promising basis for further characterization of adhesion properties of Lactobacillus and Bifidobacterium to mucosal surfaces of intestine.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 乳酸菌とムチンの相互作用 -糖鎖を介した乳酸菌の腸粘膜への付着機構-2015

    • Author(s)
      西山啓太,向井孝夫
    • Journal Title

      応用糖質科学

      Volume: 5(1) Pages: 61-65

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus gasseri SBT2055 reduces infection by and colonization of Campylobacter jejuni.2014

    • Author(s)
      Nishiyama K, Seto Y, Yoshioka K, Kakuda T, Takai S, Yamamoto Y, Mukai T.
    • Journal Title

      PLoS One

      Volume: 9(9) Issue: 9 Pages: e108827-e108827

    • DOI

      10.1371/journal.pone.0108827

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Evaluation of bifidobacterial adhesion to acidic sugar chains of porcine colonic mucins2014

    • Author(s)
      Nishiyama K, Kawanabe A, Miyauchi H, Abe F, Tsubokawa D, Ishihara K, Yamamoto Y, Mukai T.
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 78(8) Issue: 8 Pages: 1444-1451

    • DOI

      10.1080/09168451.2014.918491

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification and characterization of sulfated carbohydrate-binding protein from Lactobacillus reuteri2013

    • Author(s)
      Nishiyama K, Ochiai A, Tsubokawa D, Ishihara K, Yamamoto Y, Mukai T
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 12 Pages: e83703-e83703

    • DOI

      10.1371/journal.pone.0083703

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] シグナル配列非依存的に分泌される多機能タンパク質の役割と提示機構2013

    • Author(s)
      西山啓太、山本裕司、向井孝夫
    • Journal Title

      乳酸菌学会誌

      Volume: 24 Pages: 99-99

    • NAID

      10031184596

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation of Potential Probiotic <i>Lactobacillus</i> <i>rhamnosus</i> Strains from Traditional Fermented Mare Milk Produced in Sumbawa Island of Indonesia2012

    • Author(s)
      Shi T, Nishiyama K, Nakamata K, Aryantini NP, Mikumo D, Oda Y, Yamamoto Y, Mukai T, Sujaya IN, Urashima T, Fukuda K
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 10 Pages: 1897-1903

    • DOI

      10.1271/bbb.120385

    • NAID

      130004054008

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生きた微生物を食べて人も動物も健康に ~農学と医学領域から注目される乳酸菌2012

    • Author(s)
      西山啓太、山本裕司、向井孝夫
    • Journal Title

      ビオフィリア

      Volume: 8 Pages: 38-43

    • Related Report
      2012 Research-status Report
  • [Presentation] Lactobacillus rhamnosus 由来Mucus-binding factor の消化管粘液成分への付着における役割の評価2015

    • Author(s)
      上野慎太郎, 西山啓太, 仲又幸一, Sujaya I. Nengah, 福田健二, 浦島匡, 山本裕司, 向井孝夫
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮大学峰キャンパス(栃木県宇都宮市)
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Lactobacillus rhamnosus由来Spa線毛の糖鎖結合特性の解析2015

    • Author(s)
      西山啓太, 山本裕司, 向井孝夫
    • Organizer
      日本畜産学会第119回大会
    • Place of Presentation
      宇都宮大学峰キャンパス(栃木県宇都宮市)
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Typical signal sequence–independent export mechanism of elongation factor Tu in Lactobacillus reuteri2014

    • Author(s)
      Keita Nishiyama, Yuji Yamamoto, Mukai Takao
    • Organizer
      11th Symposium on Lactic Acid Bacteria
    • Place of Presentation
      Egmond aan Zee, the Netherlands
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Lactobacillus reutriにおけるElongation factor Tuのシグナル非依存的な分泌機構に関する研究2014

    • Author(s)
      西山啓太、南條早由利、遠藤紗耶、小菅晴春、山本裕司、向井孝夫
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ビフィズス菌由来シアリダーゼのムチン付着因子としての評価2014

    • Author(s)
      西山啓太、吹谷智、横田篤、山本裕司、向井孝夫
    • Organizer
      日本畜産学会第118回大会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Binding of EF-Tu of Lactobacillus reuteri JCM1081 to porcine gastric mucin and mucosal surface of gastrointestinal tract2013

    • Author(s)
      Nishiyama K, Kaneko H, Ochiai A, Tsubokawa D, Ishihara K, Yamamoto Y, Mukai T
    • Organizer
      IDF World Dairy Summit 2013.
    • Place of Presentation
      パンパシフィック横浜ベイホテル東急(神奈川県、横浜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] フィブロネクチン付着性乳酸菌によるCampylobacterの感染予防への活用に向けた研究2013

    • Author(s)
      西山啓太、瀬戸康幸、山本裕司、向井孝夫
    • Organizer
      日本乳酸菌学会2013年度大会
    • Place of Presentation
      北海道大学学術交流会館(北海道札幌市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Aggregation-promoting factor 1 (APF1)のフィブロネクチン結合因子としての解析

    • Author(s)
      西山啓太、遠藤沙織、中里彩季子、瀬戸康幸、中島肇、山本裕司、向井孝夫
    • Organizer
      日本畜産学会第116回大会
    • Place of Presentation
      安田女子大学(広島市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 酸素耐性乳酸菌育種方法の検討

    • Author(s)
      佐々木諒子、喜多隆文、三宅香名芽、山本裕司、向井孝夫
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Lactobacillus gasseri SBT2055由来aggregation-promoting factor1 (APF1)のフィブロネクチン結合特性の評価

    • Author(s)
      西山 啓太、遠藤 沙織、中里 彩季子、瀬戸 康幸、中島 肇、山本 裕司、向井 孝夫
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2012 Research-status Report
  • [Presentation] インドネシア・スンバワ島発酵馬乳由来Lactobacillus rhamnosusのInternalin Jの結合特性の解析

    • Author(s)
      仲又幸一、西山啓太、石塔拉、ntini Niputudesy、Arya福田健二、Sujaya I Nengah、浦島匡、山本裕司、向井孝夫
    • Organizer
      日本乳酸菌学会2012年度大会
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 抗ヘリコバクター・ハイルマニー剤2013

    • Inventor(s)
      瀬戸泰幸、東直樹、向井孝夫、中村正彦、松井英
    • Industrial Property Rights Holder
      瀬戸泰幸、東直樹、向井孝夫、中村正彦、松井英
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-12-20
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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