| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
This study was conducted to elucidate the protective effect of skim milk (SM), casein and Trolox (TRO) added to the semen diluents on maintenance of sperm cryotolerance during semen standing incubation of Agu pig.
Treatment with 0.5% SM or casein during semen standing had the beneficial effects on post-thaw sperm motility and plasmalemma integrity. Likewise, treatment with SM plus TRO increased sperm motility, and the integrity of plasmalemma and mitochondria, and decreased the amount of lipoperoxidation, whereas these phenomena were not obviously found in sperm treated with TRO only during freezing procedure. Furthermore, higher sperm penetrability to matured oocytes in vitro was maintained in post-thaw sperm treated with SM plus TRO after the beginning of semen standing.
These findings indicate that treatment with SM or casein plus TRO during semen standing incubation improved the post-thaw qualities of Agu sperm by protection of cryotolerance prior to the freezing process.
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