Development of egg-hatching assays for evaluatingTCBZ resistance of parthenogenic Fasciola sp.

• Project/Area Number: 24580420
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Iwate University
• Principal Investigator: ITAGAKI Tadashi 岩手大学, 農学部, 教授 (80203074)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: parthenogenic Fasciola / hybridyzation / Fasciola hepatica / diploid / 単為生殖型肝蛭 / トリクラベンダゾール / 虫卵孵化

Outline of Final Research Achievements

Experimental production of TCBZ-resistant parthenogenic Fasciola sp. was attempted due to hybridyzation between Fasciola hepatica and diploid parthenogenic Fasciola sp.(Fg lineage). Hybrid F1 and F2 descendants produced had aberrant meiotic division in their spermatogenesis as well as wild-type parthenogenic Fasciola sp. However, the descendants were proved to be TCBZ-susceptible due to TCBZ administration experiments, and the development of egg-hatching assay turn out not to be ture.

Research Products

• [Journal Article] Molecular phylogenetic analysis of Fasciola flukes from eastern India. (2015)
  • Author(s): Kei Hayashi, Madoka Ichikawa-Seki, Uday Kumar Mohanta, Singh T Shantikumar, Takuya Shoriki, Hiromu Sugiyama and Tadashi Itagaki
  • Journal Title: Parasitology International Volume: 64
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Morphological and molecular characterization of Eurytrema cladorchis parasitizing cattle (Bos indicus) in Bangladesh. (2015)
  • Author(s): Uday Kumar Mohanta & Madoka Ichikawa-Seki & Kei Hayashi & Tadashi Itagaki.
  • Journal Title: Parasitology Research Volume: 114
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Protection against Fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin L. (2015)
  • Author(s): Veerawat Sansri, Krai Meemon, Narin Changklungmoa, Pornanan Kueakhai, Pathanin Chantree, Pannigan Chaichanasak, Natcha Lorsuwannara, Tadashi Itagaki, Prasert Sobhon.
  • Journal Title: Vaccine Volume: 33 Pages: 1596-1601
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomal RNA among three Moniezia species isolated from ruminants in Japan. (2015)
  • Author(s): Maiko OHTORI, Mikiko AOKI and Tadashi ITAGAKI.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Pages: 105-107
  • Peer Reviewed
• [Journal Article] Molecular phylogenetic identification of Fasciola flukes in Nepal. (2014)
  • Author(s): Takuya Shoriki; Madoka Ichikawa-Seki; Bhuminand Devkota; Hari B Rana; Shiva P Devkota; Sudeep K Humagain; Tadashi Itagaki
  • Journal Title: Parasitology International Volume: 63 Pages: 758-762
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Distinct distribution of Dicrocoelium dendriticum and D. chinensis in Iwate Prefecture, Japan, and a new final host record for D. chinensis. (2014)
  • Author(s): Maiko OHTORI, Mikiko AOKI, and Tadashi ITAGAKI.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Pages: 1415-1417
  • Peer Reviewed
• [Journal Article] haracteristics and molecular phylogeny of Fasciola flukes from Bangladesh, determined based on spermatogenesis and nuclear and mitochondrial DNA analyses. (2014)
  • Author(s): Uday Kumar Mohanta, Madoka Ichikawa-Seki, Takuya Shoriki, Ken Katakura, Tadashi Itagaki.
  • Journal Title: Parasitol. Res. Volume: in press
  • Peer Reviewed
• [Journal Article] Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar. (2013)
  • Author(s): Ichikawa, M., Kondoh, D., Bawn, S., Maw, N. N., Htun, L. L., Thein, M., Gyi, A., Sunn, K., Katakura, K. and Itagaki, T.
  • Journal Title: J. Vet. Med. Sci. Volume: 75 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Immunization with recombinant leucine aminopeptidase showed protection against Fasciola gigantica in mice. (2013)
  • Author(s): Narin Changklungmoa, Pornanan Kueakhai, Suda Riengrojpitak, Kulathida Chaithirayanon, Pannigan Chaichanasak, Narin Preyavichyapugdee, Pathanin Chantree, Veerawat Sansri, Tadashi Itagaki, Prasert Sobhon
  • Journal Title: Parasitol Res Volume: 112 Pages: 3653-3659
  • Peer Reviewed
• [Journal Article] Vaccine potential of recombinant cathepsin B against Fasciola gigantica. (2013)
  • Author(s): Pathanin Chantree, Manussabhorn Phatsara, Krai Meemon, Pannigan Chaichanasak,Narin Changklungmoa, Pornanan Kueakhai, Natcha Lorsuwannarat, Kant Sangpairoj, Sineenart Songkoomkrong, Chaitip Wanichanon, Tadashi Itagaki, Prasert Sobhon
  • Journal Title: Experimental Parasitology Volume: 135 Pages: 102-109
  • Peer Reviewed
• [Journal Article] Vaccine potential of recombinant saposin-like protein 2 againstFasciolosis gigantica in mice. (2013)
  • Author(s): Pornanan Kueakhai, Narin Changklungmoa, Suda Riengrojpitak,Pannigan Chaichanasak, Krai Meemon, Kulathida Chaithirayanon,Pathanin Chantree, Veerawat Sansri, Tadashi Itagaki, Prasert Sobhon
  • Journal Title: Vaccine Volume: 31 Pages: 5518-5523
  • Peer Reviewed
• [Journal Article] アジアにおける単為生殖型肝蛭の伝播 (2013)
  • Author(s): 市川まどか、板垣 匡
  • Journal Title: 獣医寄生虫学会誌 Volume: 11 Pages: 71-79
  • NAID: 120005522952
• [Journal Article] Molecular Analysis of Aspermic Fasciola Flukes from Korea on the Basis of the Nuclear ITS1 Region and Mitochondrial DNA Markers and Comparison with Japanese Aspermic Fasciola Flukes. (2012)
  • Author(s): Ichikawa, M. and Itagaki, T.
  • Journal Title: J. Vet. Med. Sci. Volume: 74 Pages: 899-904
• [Journal Article] Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam. (2012)
  • Author(s): Nguyen, S., Amer, S., Ichikawa, M., Itagaki, T., Fukuda, Y. and Nakai, Y.
  • Journal Title: Parasite Volume: 19 Pages: 85-89
• [Journal Article] Identification of Fasciola flukes in Thailand based on their spermatogenesis and nuclear ribosomal DNA, and their intraspecific relationships based on mitochondrial DNA. (2012)
  • Author(s): Chaichanasak, P., Ichikawa, M., Sobhon, P. and Itagaki, T.
  • Journal Title: Parasitol. Int. Volume: 61 Pages: 545-549
• [Presentation] 中国チベット高原東北部におけるFasciola属の分子系統解析 (2014)
  • Author(s): 唐文強、関（市川）まどか、Mohanta Uday-Kumar, 林慶、正力拓也、板垣 匡
  • Organizer: 第60回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 岩手大学
  • Year and Date: 2014-10-18
• [Presentation] インド東部におけるFasciola属の分子系統解析 (2014)
  • Author(s): 林慶、関（市川）まどか、正力拓也、Mohanta Uday-Kumar, 杉山 広、Shantikumar S, 板垣 匡
  • Organizer: 第60回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 岩手大学
  • Year and Date: 2014-10-18
• [Presentation] 日本の異なる3地域のウシから検出されたCryptosporidium属原虫の分子学的解析 (2014)
  • Author(s): 會田淳也、関（市川）まどか、福本奈津子、浅田正嗣、八坂誠子、熊谷芳浩、西川義文、板垣 匡
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] エゾシカと家畜ウシにおける北海道産肝蛭の分子学的比較 (2014)
  • Author(s): 城間友子、関（市川）まどか、福本晋也、刈屋達也、中尾稔、板垣 匡
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Fasciola属を識別するための新規シングルコピーマーカーの開発 (2014)
  • Author(s): 正力拓也、林慶、関（市川）まどか、中尾稔、板垣 匡
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] インド産肝蛭の分子系統解析 (2014)
  • Author(s): 林慶、関（市川）まどか、正力拓也、Mohanta Uday-Kumar, 杉山 広、Shantikumar S, 板垣 匡
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Single copy geneマーカーに基づいたFasciola属の新規識別法の確立 (2014)
  • Author(s): 正力拓也、林慶、関（市川）まどか、中尾稔、板垣 匡
  • Organizer: 第8回蠕虫研究会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2014-09-06 – 2014-09-07
• [Presentation] Single copy geneマーカーを用いた中国産肝蛭の再解析：Fasciola属の種分化について (2014)
  • Author(s): 関（市川）まどか、正力拓也、林慶、板垣 匡
  • Organizer: 第8回蠕虫研究会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2014-09-06 – 2014-09-07
• [Presentation] 単為生殖型肝蛭は実験的に作出できるか？ (2014)
  • Author(s): 関（市川）まどか、島内早紀子、板垣 匡
  • Organizer: 第22回分子寄生虫ワークショップ・第12回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] Fasciola属吸虫における遺伝子組み換え技術の確立 (2014)
  • Author(s): 林慶、関（市川）まどか、正谷達謄、内藤郁慶、正力拓也、村上賢二、板垣 匡
  • Organizer: 第22回分子寄生虫ワークショップ・第12回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2014-08-31 – 2014-09-03
• [Presentation] Molecular identification and phylogeny of Fasciola flukes from Bangladesh, determined based on spermatogenesis and nuclear and mitochondrial DNA analyses (2014)
  • Author(s): Mohanta Uday-Kumar, Ichikawa-Seki Madoka, Shoriki Takuya, Katakura Ken and Itagaki Tadashi
  • Organizer: 13th International Congress of Parasitology
  • Place of Presentation: Mexico city
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] An attempt to make triploid Fasciola flukes: An experimental hybridization between F. hepatica and diploid aspermic Fasciola flukes (2014)
  • Author(s): Ichikawa-Seki M., Shimauchi, S., Fushuku, S., Fukuda, K. and Itagaki, T.
  • Organizer: 13th International Congress of Parasitology
  • Place of Presentation: Mexico city
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] 3倍体単為生殖型肝蛭の実験的作出：F. hepaticaと単為生殖型肝蛭の交雑実験 (2014)
  • Author(s): 関(市川) まどか、島内早紀子、冨宿誠吾、福田孝一、板垣 匡
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] Morphological characterization of lancet flukes (Dicrocoelium sp.) from cattle (Bos indicus) in Bangladesh. (2014)
  • Author(s): Uday Kumar Mohanta, Madoka Ichikawa-Seki, Takuya Shoriki and Tadashi Itagaki
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] アジア産肝蛭の分子系統解析：ネパールおよびフィリピン産肝蛭の分子学的解析 (2014)
  • Author(s): 正力 拓也，関 まどか，Bhuminand Devkota, Hari B. Rana, Shiva P. Devkota, Sudeep K. Humagain, Elena A. Villacorte，Pilarita T. Rivera，板垣 匡
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] 沖縄県の黒毛和種子牛におけるCryptosporidium parvumの疫学調査 (2013)
  • Author(s): 礒 雄大、関(市川) まどか、正谷達謄、板垣 匡、西川義文
  • Organizer: 第59回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 酪農学園大学研修館
• [Presentation] Characterization and molecular phylogeny of Fasciola flukes from Bangladesh on the basis of spermatogenesis, ITS1 type and nad1 haplotype. (2013)
  • Author(s): Uday Kumar Mohanta, Madoka Ichikawa-Seki, Takuya Shoriki and Tadashi Itagaki
  • Organizer: 第59回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 酪農学園大学研修館
• [Presentation] アジア産肝蛭の分子系統解析 : フィリピン産肝蛭の精子型，ITS1型およびnad1ハプロタイプの解析 (2013)
  • Author(s): 正力 拓也，関 まどか，Elena A. Villacorte，Pilarita T. Rivera，板垣 匡
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] アジア産肝蛭の分子系統解析：ミトコンドリアDNAのnadI, nad5, coxI領域に基づく解析 (2013)
  • Author(s): 阿部美智子、関(市川) まどか、板垣 匡
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] ペルー産Fasciola hepaticaの分子学的解析 (2013)
  • Author(s): 関(市川) まどか、Ortis Pedro, Cabrera Maria, 板垣 匡
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] Fasciola hepaticaと単為生殖型肝蛭2倍体の実験交雑により作出した3倍体肝蛭について (2013)
  • Author(s): 島内早紀子、関(市川) まどか、板垣 匡
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] Origin and spread of aspermic Fasciola sp. in Asia. (2013)
  • Author(s): Tadashi Itagaki, Madoka Ichikawa-Seki, Takuya Shoriki, Pannigan Chaichanasak, Peng Mao and Keisuke Sakaguchi
  • Organizer: Joint International Tropical Medicine Meeting 2013.
  • Place of Presentation: Centra Grand & Bangkok Convention Centre
• [Presentation] Molecular characterization and phylogenetic analysis of Fasciola hepatica from Cajamarca, Peru. (2013)
  • Author(s): Madoka Ichikawa-Seki, Pedro Ortiz, Maria Cabrera, Tadashi Itagaki
  • Organizer: 24th International Conference of the World Association for the Advancement of veterinary Parasitology
  • Place of Presentation: Perth Convention Exhibition Centre Western Australia
• [Presentation] Molecular detection and characterization of Cryptosporidium species in private-household dogs and pet shop puppies in Japan. (2013)
  • Author(s): Naoyuki Itoh, Yoshino Oohashi, Madoka Ichikawa-Seki, Tadashi Itagaki, Yoichi Ito, Kazutaka Kanai, Seishiro Chikazawa, Yasutomo Hori, Fumio Hoshi, Seiichi Higuchi.
  • Organizer: 24th International Conference of the World Association for the Advancement of veterinary Parasitology
  • Place of Presentation: Perth Convention Exhibition Centre Western Australia
• [Presentation] Development of nested PCR specific to bovine Eimeria species. (2013)
  • Author(s): Takuya Kokuzawa, Madoka Ichikawa-Seki, Tadashi Itagaki
  • Organizer: 24th International Conference of the World Association for the Advancement of veterinary Parasitology
  • Place of Presentation: Perth Convention Exhibition Centre Western Australia
• [Presentation] ネパール産肝蛭の精子型，ITS1型およびnad1型の解析 (2012)
  • Author(s): 正力拓也，市川まどか，Bhuminand Devkota，Hari B. Rana，Shiva P. Devkota，Sudeep K. Humagai， 板垣 匡
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学、盛岡、岩手
• [Presentation] アジアに分布するFasciola giganticaの系統関係の解明: ラオス産肝蛭の分子学的解析 (2012)
  • Author(s): 市川まどか, Luiza Rast, 正力拓也, 板垣 匡
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学、盛岡、岩手
• [Presentation] アジアに分布するFasciola spp.の系統関係の解明 (2012)
  • Author(s): 市川まどか、正力拓也、Luiza Rast、板垣 匡
  • Organizer: 第58回日本寄生虫学会日本衛生動物学会北日本合同大会
  • Place of Presentation: 旭川市勤労者福祉総合センター、旭川、北海道
• [Presentation] 無精子型肝蛭の精巣におけるアポトーシスの検出 (2012)
  • Author(s): 郷右近賢司、市川まどか、近藤大輔、板垣 匡
  • Organizer: 第58回日本寄生虫学会日本衛生動物学会北日本合同大会
  • Place of Presentation: 旭川市勤労者福祉総合センター、旭川、北海道
• [Presentation] Three distinct subpopulations of Fasciola gigantica in Zambia (2012)
  • Author(s): Madoka Ichikawa, Shotaro Yuki, Jun Yasuda, Tadashi Itagaki
  • Organizer: The 15th Japan-Korea Parasitologists’ Seminar, Miyazaki.
  • Place of Presentation: Miyazaki Aoshima Palm Beach Hotel,Miyazaki, Japan