Importance of rabies virus P protein isoforms in evasion of innate immunity and pathogenicity

• Project/Area Number: 24580424
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Gifu University
• Principal Investigator: ITO Naoto 岐阜大学, 応用生物科学部, 准教授 (20334922)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 狂犬病 / 狂犬病ウイルス / P蛋白質 / 病原性 / 国際研究者交流 / オーストラリア

Outline of Final Research Achievements

Rabies virus P gene encodes several P protein isoforms (P1-P3) in the same reading frame. While P1 protein functions as a co-factor of viral RNA polymerase, little is known about the functions of P2 and P3 proteins. In this study, the importance of these P protein isoforms in viral pathogenicity was examined. The results obtained from experiments with viral mutants overexpressing or lacking each isoform suggest that P1 protein is mainly related to the viral neuropathogenicity.

Research Products

• [Journal Article] Involvement of the rabies virus phosphoprotein gene in neuroinvasiveness (2013)
  • Author(s): Yamaoka, S, Ito, N, Ohka, S, Kaneda, S, Nakamura, H, Agari, T, Masatani, T, Nakagawa, K, Okada, K, Okadera, K, Mitake, H, Fujii, T, Sugiyama, M
  • Journal Title: J. Virol Volume: 87(22) Issue: 22 Pages: 12327-38
  • DOI: 10.1128/jvi.02132-13
  • Peer Reviewed
• [Presentation] 狂犬病ウイルスの神経病原性および神経侵入性におけるP蛋白質アイソフォームの重要性 (2015)
  • Author(s): 岡田和真、伊藤直人、中川賢人、岡寺康太、三竹博道、杉山誠
  • Organizer: 第14回狂犬病研究会
  • Place of Presentation: 国立感染症研究所戸山庁舎（東京都新宿区）
  • Year and Date: 2015-04-03
• [Presentation] 狂犬病ウイルスの病原性に重要なP蛋白質アイソフォームの同定 (2014)
  • Author(s): 岡田和真、伊藤直人、中川賢人、岡寺康太、三竹博道、杉山誠
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 狂犬病ウイルスP蛋白質アイソフォーム（P3～P5）は病原性に関与する (2013)
  • Author(s): 岡田和真、伊藤直人、山岡理子、岡寺康太、杉山誠
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 狂犬病ウイルスの病原性におけるP蛋白質アイソフォームの重要性 (2012)
  • Author(s): 岡田和真、伊藤直人、中川敬介、山岡理子、岡寺康太、杉山誠
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学