| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Lungs from mice treated with yellow dust(Kosa) and gold nanoparticles showed inflammation with cytokines (IL-6, TNF-α) and oxidative stress markers (Cu/Zn SOD, iNOS) in alveolar macrophages, type I alveolar epithelial cells, and endothelial cells at the alveolar walls. Electron microscopy revealed a destruction of the alveolar walls with an increased number of endocytic vesicles containing nanoparticles in the cytoplasm of both type I epithelial cells and endothelial cells, suggesting that translocation of the nanoparticles may be enhanced in the lung tissues with acute inflammatory changes by Kosa.
An increase of endocytic vesicles in the cytoplasm of clathrin positive syncytiotrophoblasts and caveolin-1 positive fetal endothelial cells in the maternal fetal barrier of pregnant mice treated with gold nanoparticles, suggesting that intravenous administration of nanoparticles may upregulate clathrin- and caveolin-mediated endocytosis at the maternal fetal barrier in placenta.
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