Effects of yellow dust (Kosa) and nano-particles on health: localization of nanoparticles in the body after co-exposure of the particles

• Project/Area Number: 24580425
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Azabu University
• Principal Investigator: SHIMADA Akinori 麻布大学, その他部局等, 教授 (20216055)
• Research Collaborator: RATTANAPINYOPITUK Kasem
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 黄砂 / ナノ粒子 / 空気血液関門 / エンドサイトーシス / カベオリン / クラスリン / 血液胎盤関門 / サイトカイン / 活性酸素種 / 肺傷害 / カベオラ小胞 / 肺 / 肉芽腫 / 急性 / 慢性 / 炎症 / マウス / 気管内投与

Outline of Final Research Achievements

Lungs from mice treated with yellow dust(Kosa) and gold nanoparticles showed inflammation with cytokines (IL-6, TNF-α) and oxidative stress markers (Cu/Zn SOD, iNOS) in alveolar macrophages, type I alveolar epithelial cells, and endothelial cells at the alveolar walls. Electron microscopy revealed a destruction of the alveolar walls with an increased number of endocytic vesicles containing nanoparticles in the cytoplasm of both type I epithelial cells and endothelial cells, suggesting that translocation of the nanoparticles may be enhanced in the lung tissues with acute inflammatory changes by Kosa.
An increase of endocytic vesicles in the cytoplasm of clathrin positive syncytiotrophoblasts and caveolin-1 positive fetal endothelial cells in the maternal fetal barrier of pregnant mice treated with gold nanoparticles, suggesting that intravenous administration of nanoparticles may upregulate clathrin- and caveolin-mediated endocytosis at the maternal fetal barrier in placenta.

Research Products

• [Journal Article] Demonstration of the clathrin- and caveolin-mediated endocytosis at the maternal-fetal barrier in mouse placenta after intravenous administration of gold nanoparticles. (2014)
  • Author(s): Rattanapiyopituki K, Shimada A, Morita T, Sakurai M, Asano A, Hasegawa T, Inoue K, Takano H.
  • Journal Title: J Vet Med Sci Volume: 76 Pages: 377-387
  • NAID: 130003382385
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ultrastructural changes in the air-blood barrier in mice after intratracheal instillation of Asian sand dust and gold nanoparticles. (2013)
  • Author(s): Rattanapinyopituk K, Shimada A, Morita T, Togawa M, Hasegawa T, Seko Y, Inoue K, Takano H.
  • Journal Title: Exp Toxicol Pathol Volume: 65 Issue: 7-8 Pages: 1043-1051
  • DOI: 10.1016/j.etp.2013.03.003
  • Peer Reviewed
• [Journal Article] Pathological Study of Chronic Pulmonary Toxicity Induced by Intratracheally Instilled Asian Sand Dust (Kosa) (2013)
  • Author(s): Naota M, Shiotsu S, Shimada A, Kohara Y, Morita T, Inoue K, Takano H.
  • Journal Title: Toxicologic Pathology Volume: 41 Issue: 1 Pages: 48-62
  • DOI: 10.1177/0192623312452490
  • Peer Reviewed
• [Presentation] Ultrastructural changes in the air-blood barrier in mice after intratracheal instillations ofAsian sand dust and gold nanoparticles (2013)
  • Author(s): Rattanapinyopituk Kasem、島田章則、森田剛仁
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学