Function of vitamin B12 on neural cell differentiation and an insight on cognitive symptom.

• Project/Area Number: 24580428
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: TAKENAKA Shigeo 大阪府立大学, 生命環境科学研究科(系), 准教授 (10280067)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ビタミンB12 / 神経 / 分化 / レイチノイン酸 / RNAi / 神経細胞 / メチルマロン酸 / エネルギー代謝 / アデノシルB12 / SH-SY5 / GCMS / 神経細胞分化

Outline of Final Research Achievements

Vitamin B12 (B12) is an essential nutrient and its serum content is correlated with progression of cognitive symptom as seen in Alzheimer’s and Parkinson’s disease. Here I examined effects of B12 metabolizing protein down regulation using RNA interference technique on differentiation of SH-SY5Y cell by retinoic acid to have an insight on the relation between the symptom and B12. The down regulation of the genes decreased the rate of differentiation without any significant change of metabolite profile. Mitochondrial dysfunction and oxidative stress which happen under B12 deficient condition were not observed in the cells. These indicate that B12 metabolism has a part in protective function on differentiation of neural cell in unknown pathway.

Research Products

• [Journal Article] Profiling of plasma metabolites in canine oral melanoma using gas chromatography-mass spectrometry. (2015)
  • Author(s): Kawabe M, Baba Y, Tamai R, Yamamoto R, Komori M, Mori T, Takenaka S.
  • Journal Title: J Vet Med Sci. Volume: 77
  • NAID: 130005094686
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oxidation of Acenaphthene and Acenaphthylene by Human Cytochrome P450 Enzymes. (2015)
  • Author(s): Shimada T, Takenaka S, Murayama N, Yamazaki H, Kim JH, Kim D, Yoshimoto FK, Guengerich FP, Komori M.
  • Journal Title: Chem Res Toxicol. Volume: 28 Issue: 2 Pages: 268-278
  • DOI: 10.1021/tx500505y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gas chromatography-mass spectrometry-based metabolic profiling of cerebrospinal fluid from epileptic dogs. (2014)
  • Author(s): Hasegawa T, Sumita M, Horitani Y, Tamai R, Tanaka K, Komori M, Takenaka S.
  • Journal Title: J Vet Med Sci. Volume: 76 Pages: 517-522
  • NAID: 130003382409
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Profiling of serum metabolites in canine lymphoma using gas chromatography mass spectrometry. (2014)
  • Author(s): Tamai R, Furuya M, Hatoya S, Akiyoshi H, Yamamoto R, Komori Y, Yokoi S, Tani K, Hirano Y, Komori M, Takenaka S.
  • Journal Title: J Vet Med Sci. Volume: 76 Pages: 1513-1518
  • NAID: 130004677727
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gas Chromatography-Mass Spectrometry-Based Metabolic Profiling of Cerebrospinal Fluid from Epileptic Dogs (2014)
  • Author(s): Hasegawa T, Sumita M, Horitani Y, Tamai R, Tanaka K, Komori M, Takenaka S.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 4 Pages: 517-522
  • DOI: 10.1292/jvms.13-0520
  • NAID: 130003382409
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] 犬のリンパ腫における血清代謝物プロファイル (2015)
  • Author(s): 田舞理央、竹中重雄、他9名
  • Organizer: 獣医内科学アカデミー
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-02-20 – 2015-02-21
• [Presentation] 先天性ビタミンB12代謝異常症遺伝子群のRNAiによるヒト神経芽細胞腫の分化抑制 (2014)
  • Author(s): 馬場雄大、山木降裕、田舞理央、小森雅之、竹中重雄
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] アデノシルコバラミン合成酵素RNAiによる神経細胞の分化抑制 (2014)
  • Author(s): 竹中重雄、森田大樹、馬場雄太、田舞理央、小森雅之、渡辺文雄、中野長久
  • Organizer: 日本ビタミン学会
  • Place of Presentation: 姫路商工会議所
  • Year and Date: 2014-06-13 – 2014-06-14
• [Presentation] ラット脳における補酵素型ビタミンB12の日内変化 (2013)
  • Author(s): 竹中重雄、伊佐桃子、梶村俊介、森田大樹、田舞理央、田中勝啓、小森雅之、桂博美、渡辺文雄、中野長久
  • Organizer: 日本ビタミン学会 第65回大会
  • Place of Presentation: 一橋大学・一橋講堂
• [Presentation] アデノシルコバラミン合成酵素RNAiによる (2013)
  • Author(s): 森田大樹、田舞理央、梶村俊介、馬場雄大、小森雅之、竹中重雄
  • Organizer: 日本獣医学会第156回学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] ラット脳におけるビタミンB12とB12依存酵素メチルマロニルCoAムターゼとメチオニンシンターゼの日内変動 (2013)
  • Author(s): 梶村 俊介，伊佐 桃子，森田 大樹，馬場 雄大，田舞 理央、小森 雅之，竹中 重雄
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] ビタミンB12代謝異常症原因遺伝子MMABノックダウンによる細胞代謝への影響 (2012)
  • Author(s): 竹中重雄, 楠本一仁, 鈴來佑介, 角田真穂, 伊佐桃子, 田舞理央, 田中勝啓, 小森雅之, 渡辺文雄, 中野長久
  • Organizer: 日本ビタミン学会
  • Place of Presentation: 長良川国際会議場