Function of novel pituitary transcirption facotr PRX in the development of the pituitary galnd and teh vasculogenesis
Project/Area Number |
24580435
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Basic veterinary science/Basic zootechnical science
|
Research Institution | Meiji University |
Principal Investigator |
KATO Takako 明治大学, 公私立大学の部局等, 研究員 (90445859)
|
Co-Investigator(Renkei-kenkyūsha) |
KATO Yukio 明治大学, 農学部, 教授 (30114177)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 遺伝子 / 発現制御 / 内分泌学 / 生殖 / 細胞・組織 / 血管形成 / 分化 |
Outline of Final Research Achievements |
This study aimed to understand role of the novel transcription factors, PRX1 and PRX2. Using generated specific antibodies for PRX1 and PRX2, we demonstrated their presence in the pituitary stem/progenitor cells and in the extrapituitary cells invaded from surrounding mesenchyme and suggest that PRX1 and PRX2 are involved in pituitary organogenesis and vasculogenesis. In addition, we have found that TtT / GF cell derived from pituitary tumors expresses PRX1 and PRX2 and exhibits stemness with expressing several stem cell markers, showing a useful model cell to clarify their functions in molecular level.
|
Report
(4 results)
Research Products
(94 results)
-
[Journal Article] PRRX1- and PRRX2-positive mesenchymal stem/progenitor cells are involved in vasculogenesis during rat embryonic pituitary development.2015
Author(s)
Higuchi, M., Yoshida, S., Ueharu, H., Chen, M., Kato, T. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 358
Issue: 2
Pages: 557-565
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] GFP-expressing S100β-positive cells of the rat anterior pituitary differentiate into hormone-producing cells.2014
Author(s)
Higuchi, M., Kanno, N., Yoshida, S., Ueharu, H., Chen, M., Yako, H., Shibuya, S., Sekita, M., Tsuda, M., Mitsuishi, H., Kato, T. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 357
Pages: 767-779
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] PRRX1 and PRRX2 distinctively participate in pituitary organogenesis and cell supply system.2014
Author(s)
Higuchi, M., Yoshida, S., Ueharu, H., Chen, M., Kato, T. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 357
Pages: 323-335
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Expression of chemokine CXCL10 in dendritic cell-like S100β-positive cells in rat anterior pituitary gland.2014
Author(s)
Horiguchi, K., Fujiwara, K., Higuchi, M., Yoshida, S., Tsukada, T., Ueharu, H., Hasegawa, R., Takigami, S., Ohsako, S., Yashiro, T., Kato, T. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 357
Pages: 757-765
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Isolation of dendritic cell-like S100β-positive cells in rat anterior pituitary gland.2014
Author(s)
Horiguchi, K., Fujiwara, K., Yoshida, S., Higuchi, M., Tsukada, T., Kanno, N., Yashiro, T., Tateno, K., Osako, S., Kato, T. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 357
Pages: 757-765
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Proton receptor GPR68 expression in dendritic cell-like S100β-positive cells of rat anterior pituitary gland: GPR68 induces interleukin-6 gene expression in extracellular acidification.2014
Author(s)
Horiguchi, K., Higuchi, M., Yoshida, S., Nakakura, T., Tateno, K., Hasegawa, R., Osako, S., Kato, T. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 358
Pages: 515-525
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Expression of kruppel-like factor 6, KLF6, in rat pituitary stem/progenitor cells and its regulation of the PRRX2 gene.2014
Author(s)
Ueharu, H., Higuchi, M., Nishimura, N., Yoshida, S., Shibuya, S., Sensui, K., Kato, T. and Kato, Y.
-
Journal Title
J Reprod Dev
Volume: 60
Pages: 304-311
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF.2014
Author(s)
Yoshida, S., Higuchi, M., Ueharu, H., Nishimura, N., Tsuda, M., Nishihara, H., Mitsuishi, H., Kato, T. and Kato, Y.
-
Journal Title
J Reprod Dev
Volume: 60
Pages: 295-303
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Localization of a juxtacrine factor ephrin-B2 in the pituitary stem/progenitor cell niches throughout life.2014
Author(s)
Yoshida, S., Kato, T., Chen, M., Higuchi, M., Ueharu, H., Nishimura, N. and Kato, Y.
-
Journal Title
Cell Tissue Res
Volume: 359
Pages: 755-766
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Molecular cloning of rat and porcine retina-derived POU domain factor 1 (POU6F2) from pituitary cDNA library.2014
Author(s)
Yoshida, S., Ueharu, H., Higuchi, M., Horiguchi, K., Nishimura, N., Shibuya, S., Mitsuishi, H., Kato, T. and Kato, Y.
-
Journal Title
J Reprod Dev
Volume: 60
Pages: 288-294
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
[Journal Article] Detection of human herpesviruses (HHVs) in semen of human male infertile patients.2013
Author(s)
Chen M, Cai LY, Kanno N, Kato T, Lu J, Jin F, Wang H, Sekita M, Higuchi M, Yoshida S, Yako H, Ueharu H, Izumi S-I, Kato Y.
-
Journal Title
Journal of Reproduction and Development
Volume: 59
Pages: 457-462
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Characterization of a pituitary-tumor-derived cell line, TtT/GF, that expresses Hoechst efflux ABC transporter subfamily G2 and stem cell antigen 1.2013
Author(s)
Mitsuishi H, Kato T, Chen M, Cai LY, Yako H, Higuchi M, Yoshida S, Kanno N, Ueharu H, Kato Y.
-
Journal Title
Cell and Tissue Research
Volume: 354
Pages: 563-572
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 下重体前葉内S100タンパク質陽性細胞から分泌され るケモカインCXCL10のlFN-γによる制御.2014
Author(s)
堀口幸太郎, 吉田彩舟, 藤原研, 樋口雅司, 塚田岳大, 加藤たか子, 舘野こずえ, 長谷川留美, 瀧上潤, 大迫俊二, 屋代隆、加藤幸雄.
Organizer
第40回日本神経内分泌学会学術集会
Place of Presentation
都道府県会館
Year and Date
2014-10-31 – 2014-11-02
Related Report
-
-
-
-
-
-
-
[Presentation] Invasion of S100β-positive cells into pituitary gland during embryonic period.2014
Author(s)
H. Yako, K. Horiguchi, M. Higuchi, K. Fujiwara, S. Yoshida, M. Chen, N. Kanno, H. Ueharu, T. Kato, T. Yashiro and Y. Kato.
Organizer
World Congress of Reproductive Biology 2014
Place of Presentation
Edinburgh, UK
Year and Date
2014-09-02 – 2014-09-04
Related Report
-
-
-
-
-
[Presentation] 樹状細胞様S100タンパク質陽性細胞から分泌されるケモカインCXCL10の機能解析.2014
Author(s)
堀口幸太郎, 吉田彩舟, 藤原研, 樋口雅司, 塚田岳大, 加藤たか子, 舘野こずえ, 長谷川留美, 瀧上潤, 大迫俊二, 屋代隆、加藤幸雄.
Organizer
第29回日本下垂体研究会学術集会
Place of Presentation
八王子セミナーハウス
Year and Date
2014-08-08 – 2014-08-10
Related Report
-
-
-
-
-
[Presentation] S100b-positive cells (follicle satellite cell) drove from two different origins in the pituitary.2013
Author(s)
Hideji Yako, Masashi Higuchi, Saishu Yoshida, Kotaro Horiguchi, Ken Fujiwara, Naoko Kanno, Mo Chen, Hiroki Ueharu, Mituyoshi Tsuda, Takako Kato, Takashi Yashiro, Yukio Kato.
Organizer
The 95th endocrine Society Meeting
Place of Presentation
San Francisco
Related Report
-
[Presentation] Novel pituitary transcription factors, Prrx 1 and Prrx2, may be involved in the pituitary organogenesis under differential gene regulation by several transcription factors.2013
Author(s)
Hiroki Ueharu, Shiori Shibuya, Mitsuyoshi Tsuda, Masayo Sekita, Masashi Higuchi, Saishu Yoshida, Hideji Yako, Naoko Kanno, Mo Chen, Takako Kato, Yukio Kato.
Organizer
The 95th endocrine Society Meeting
Place of Presentation
San Francisco
Related Report
-
[Presentation] Temporospatial localization pattern of PRRX1 and PRRX2 suggests essential roles in differentiation into hormone-producing cell and vascular cells during rat pituitary organogenesis.2013
Author(s)
Masashi Higuchi, Saishu Yoshida, Mitsuyoshi Tsuda, Shiori Shibuya, Hiroki Ueharu, Masayo Sekita, Hideji Yako, Naoko Kanno, Mo Chen, Takako Kato, Yukio Kato.
Organizer
The 95th endocrine Society Meeting
Place of Presentation
San Francisco
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-