| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We incubated three avian influenza viruses, H8N4, H10N2, and H12N5, in MDCK canine cells and got each mutated virus which had the ability to grow over 100-times better than parental one. We analyzed all nucleotide sequences of parental and mutated viruses. By making reassortants between parental and mutated viruses, it was found that PB2 and HA was responsible for increased growth ability. All three viruses had mutations in PB2 and HA. By making reassortants with a single mutation found in PB2 and HA, we identified mutations associated increased growth ability. A mutation in PB2 increased viral polymerase activity. Mutations in HA increased viral fusion activity after endocytosis.
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