Surveillance on high pathogenic avian influenza viruses among migratory birds in Vietnam

• Project/Area Number: 24580446
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo (2013-2014); Nagasaki University (2012)
• Principal Investigator: HOTTA Kozue 東京大学, 農学生命科学研究科, 助教 (70466192)
• Co-Investigator(Kenkyū-buntansha): ITO Toshihiro 鳥取大学, 農学部・獣医学科, 教授 (00176348)
• Co-Investigator(Renkei-kenkyūsha): TAKAKUWA Hiroki 京都産業大学, 総合生命科学部, 教授 (20449507)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 獣医学 / 獣医公衆衛生学 / 疫学調査 / ベトナム / 鳥インフルエンザウイルス / 渡り鳥 / ベトナム国立衛生疫学研究所 / 国際情報交換

Outline of Final Research Achievements

To know the prevalence of avian influenza viruses including the high pathogenic avian influenza virus H5N1 among migratory birds in the northern Vietnam, we collected feces of migratory birds from 2012 to 2014 and samples were inoculated into the chicken embryo to isolate the viruses. The isolated viruses were analyzed the sequences and determined the subtypes. About the classification of migratory birds, we extracted DNA from feces and amplified the mitochondrial DNA by PCR. Amplified mitochondrial DNA were sequenced and then searched the data bank (Consortium for the Barcode of life).

Research Products

• [Journal Article] A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity (2014)
  • Author(s): Watanabe Y, Ito T, Ibrahim MS, Arai Y, Hotta K, Phuong HVM, Hang NLK Mai LQ, Soda K, Yamaoka M, Poetranto ED, Wulandari L, Hiramatsu H, Daidoji T, Kubota-Koketsu R, Sriwilaijaroen N, Nakaya T, Okuno Y, Takahashi T, Suzuki T, Ito T, Hotta H, Yamashiro T, Hayashi T, Morita K, Ikuta K, Suzuki Y
  • Journal Title: Biosens Bioelectron Volume: 65 Pages: 211-219
  • DOI: 10.1016/j.bios.2014.10.036
  • Peer Reviewed / Open Access / Acknowledgement Compliant