| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Transmission of Campylobacter to humans is considered to be consumption of chicken meat that has been contaminated during processing. In the present study, adhesion factors in both of Campylobacter and chicken skin have been examined. Mutants which lack cell surface materials were constructed by natural transformation-mediated allelic exchange. The mutagenesis studies clearly indicated that capsular polysaccharide expression on the cell surface played a major role in the attachment on skin surfaces. Then, the numbers of Campylobacter attached to the skin before and after treatment with sodium hydroxide were determined. In comparison with pretreated skin, the mean reductions obtained by the alkaline treatment exceeded the one log CFU per 10 g back skin, suggesting that adhesion factors on the skin may be extracted by this treatment. An adhesion factor showing about 100-150 KDa was detected by a two-dimensional SDS-PAGE and over-lay assay.
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