Development of diagnostic methods for early and prognosis of mycobacterial infection

• Project/Area Number: 24580448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: University of Miyazaki
• Principal Investigator: GOTO Yoshitaka 宮崎大学, 農学部, 教授 (30142136)
• Co-Investigator(Kenkyū-buntansha): HAGA Takeshi 東京大学, 大学院農学生命科学研究科, 准教授 (20315360)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 豚 / 抗酸菌 / 感染症 / 診断 / サイトカイン / mycobacterium / avium / hominissuis / 抗酸菌症 / Mycobacterium / マウス / Mycobacterium avium / 遺伝子解析 / 診断法

Outline of Final Research Achievements

Mycobacteriosis of pigs caused by Mycobacterium avium subsp. by hominissuis (Mah) is an infectious disease which forms the granulomatous lesions similar to tuberculosis in visceral organs. So far only discovery by the naked eye of veterinarians in slaughterhouses has become a decisive diagnosis and such infectious organs have been discarded. Therefore, it appears to be necessary to develop a new diagnostic method. We has been looking for the cytokines which were induced by Mah infection. Qualitative or quantitative assay of these cytokines seems to be useful for diagnosis of porcine mycobacterial infection. We found several cytokines as candidates. Types and amounts of cytokines produced varied with the time of Mah infection and progress of the disease. We are currently being developed for diagnosis of infectious conditions utilizing the difference in the inducing pattern of these cytokines in the infected pigs.

Research Products

• [Presentation] 抗酸菌感染症豚の脾細胞から分泌されるサイトカイン (2014)
  • Author(s): 岡本理、渡邉真治、後藤義孝
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] レッサーパンダのＴ細胞リンパ腫を併発したMycobacterium gastri感染症の一例 (2014)
  • Author(s): 福家直幸、平井卓也、山口良二、後藤義孝
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 抗酸菌症の豚から分離されるM.avium subsp. hominissuisの多様性について (2013)
  • Author(s): 水谷友香、渡邉真治、後藤義孝
  • Organizer: 第158回日本獣医学会総会
  • Place of Presentation: 岐阜
• [Presentation] 酸性条件下におけるMycobacterim aviumのarcA mRNA発現誘導の解析 (2013)
  • Author(s): 小川翔大、小川賢二、大原直也、後藤義孝、瀧井猛将
  • Organizer: 第８６回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Mycobacterium avium の低ｐH環境下でのアンモニア産生について (2013)
  • Author(s): 小川翔大、瀧井猛将、花村菜月、宮田江里加、筑比地慧、山本龍二、 堀田康弘、伊藤佐生智，小川賢 二，八木哲也，西森敬，大原直也，藤原永年，前田伸司， 山崎利雄，後藤義孝、小野嵜菊夫
  • Organizer: 第83回実験結核研究会
  • Place of Presentation: 東京