Investigation of pathogenic mechanisms of new pathogenic factor NetB from Clostridium perfringens that causes avian necrotic entelitis.

Research Project

Project/Area Number	24580449
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied veterinary science
Research Institution	Osaka Prefecture University
Principal Investigator	MUKAMOTO Masafumi 大阪府立大学, 生命環境科学研究科(系), 教授 (80231629)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	細菌毒素 / 腸炎 / ニワトリ / 感染症 / ウエルシュ菌 / NetB
Outline of Final Research Achievements	It was clear that strains of possessing netb in C. perfringens type A were widely distributed in Japan and were infectious in healthy chickens. Though netb exist on a conjugative plasmid, netb was not easily transferred among cells and strains possessing netb that are rampant throughout the same areas were single or a small number. NetB is a pore forming toxin that possessed high specific pathogenic activities against the order of pheasants containing chickens. NetB was bound with non-raft regions on target cell membranes without participation of cholesterol. NetB polymerized among neighboring monomer molecules formed oligomer formation. In this study I made a part of molecular movement from binding to cells to pore formation of NetB clear.