| Project/Area Number |
24580451
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OSAWA Yoshiaki 北海道医療大学, 薬学部, 准教授 (20415558)
KANNO Toru 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 主席研究員 (80355205)
INOUE Emi 北海道医療大学, 薬学部, 助教 (80433423)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | enzootic bovine leucosis / bovine leukemia virus / control / Tax / colostrum / 地方病性牛白血病 / 牛白血病ウイルス / Taxタンパク質 / RNaseH / 逆転写酵素 / ワクチン / 発症前診断 / 弱毒生ワクチン / ドミナント・ネガティブ効果 / がん関連遺伝子 / 転写調節 / 母子感染 / 診断 |
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| Outline of Final Research Achievements |
Bovine leukemia virus (BLV) is a causative agent of enzootic bovine leucosis. Cattle infected with the virus carrying Tax with Pro at amino acid position 233 (P233-Tax) had 2-year longer incubation period for developing tumors than those with the virus carrying L233-Tax. P233-Tax significantly downregulated the human oncogene jun and had poorer transactivation activity against cdk4 promoter than L233-Tax. P233-Tax expressing cells also formed smaller tumors in murine xenograft study than L233-Tax expressing cells. It is, therefore, considered that Tax descrimination might be useful for determining the prognosis of cattle with inapparent infections of BLV.
Freezing treatment on colostrum inactivated the infectivity of BLV-infected lymphocytes to prevent the transmission of BLV among neonatal calves.
These findings are expected to be useful for the control of enzootic bovine leucosis.
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