Elucidation of the molecular epidemiology of bat-associated rabies virus isolates in Brazil

• Project/Area Number: 24580453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Nihon University
• Principal Investigator: SAKAI Takeo 日本大学, 生物資源科学部, 名誉教授 (50147667)
• Co-Investigator(Kenkyū-buntansha): ITOU Takuya 日本大学, 生物資源科学部, 准教授 (20307820) ; SUZUKI Yuki 日本大学, 生物資源科学部, 助教 (30712492)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 狂犬病ウイルス / 分子疫学 / コウモリ / 遺伝子解析 / ブラジル / 野生動物 / 狂犬病 / ウイルス弱毒株 / 国際研究者交流 / サル

Outline of Final Research Achievements

Molecular epidemiological analysis using Brazilian field rabies virus isolates revealed the following results;
1) The useful virus RNA extraction and storage method was established using FTA card. 2) Distribution area and transmission route of the bat-related RABV in the northeastern Brazil were elucidated. 3) Phylogenetic analyses showed that the BRmk1358 strain, isolated from a tufted capuchin monkey in cetntral Brazil, formed a lineage distant from that of marmoset rabies virus within the bat-related rabies virus cluster.

Research Products

• [Journal Article] Establishing conditions for the storage and elution of rabies virus RNA using FTA^® cards (2015)
  • Author(s): Sakai T, Ishii A, Segawa T, Takagi Y, Kobayashi Y, Itou T
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 4 Pages: 461-465
  • DOI: 10.1292/jvms.14-0227
  • NAID: 130005067018
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Easy-to-use rapid gene amplification method for direct detection of RNA and DNA viruses in sera and feces from various animals (2014)
  • Author(s): Segawa T, Kobayashi Y, Sase Y, Itou T, Suzuki M, Endoh T, Nakanishi T, Sakai
  • Journal Title: J Virol Methods. Volume: 201C Pages: 31-37
  • DOI: 10.1016/j.jviromet.2014.01.019
  • Peer Reviewed
• [Journal Article] Isolation of a phylogenetically distinct rabies virus from a tufted capuchin monkey (Cebus apella) in Brazil. (2013)
  • Author(s): Kobayashi Y, Sugimoto K, Mochizuki N, Segawa T, Itou T, Carvalho AA, Nociti DP, Mello RM, Santos AK, Ito FH, Sakai T.
  • Journal Title: Virus Res. Volume: 178 Issue: 2 Pages: 535-538
  • DOI: 10.1016/j.virusres.2013.09.020
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of livestock rabies viruses isolated in the northeastern Brazilian states of Paraiba and Pernambuco from 2003-2009. (2012)
  • Author(s): Mochizuki N, Kawasaki H, Silva ML, Afonso JA, Itou T, Ito FH, Sakai T.
  • Journal Title: BMC Res Notes. Volume: 5 Issue: 1 Pages: 32-32
  • DOI: 10.1186/1756-0500-5-32
  • Peer Reviewed
• [Presentation] ブラジルの吸血および食果コウモリ由来狂犬病ウイルスの分子系統解析 (2014)
  • Author(s): 日下部美帆、小林由紀、Fumio HF. Ito、酒井健夫、伊藤琢也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学高等教育推進機構
  • Year and Date: 2014-09-10
• [Presentation] ブラジルのフサオマキザル(Cebus apella)から分離された狂犬病ウイルスの分子系統解析 (2013)
  • Author(s): 小林由紀，杉本かほり，望月信之，瀬川太雄，伊藤琢也，Adolorata A.B. Carvalho，Darci P. Nociti，Rosane M. Mello，Anna K.R.A. Santos，Fumio H. Ito，酒井健夫
  • Organizer: 第13回人と動物の共通感染症研究会
  • Place of Presentation: 国立感染症研究所
• [Presentation] 狂犬病ウイルスRNA保存のためのFTAカード使用法の最適化 (2013)
  • Author(s): 石井亜矢子，瀬川太雄，小林由紀，伊藤琢也，Ito H. Fumio，酒井健夫
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] 抽出作業を省いた簡易・迅速なウイルス遺伝子検出法の開発 (2013)
  • Author(s): 佐瀬友紀奈，瀬川太雄，鈴木美和，小林由紀，遠藤智子，伊藤琢也，酒井健夫
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] ブラジルのフサオマキザルから分離された狂犬病ウイルスの分子疫学的解析 (2013)
  • Author(s): 杉本かほり, 小林由紀, 伊藤琢也, Calvalho AB, Ito FH, 酒井健夫
  • Organizer: 第12回狂犬病研究会
  • Place of Presentation: 国立感染症研究所
• [Book] Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 1. (2014)
  • Author(s): Itou T, Markotter W, Nel LH., (Eds. Rupprecht C. and Nagarajan T.)
  • Total Pages: 350
  • Publisher: Academic Press/Elsevier Inc.