The role of Babesia gibsoni heat shock protein 90 on the proliferation and the drug resistance.
| Project/Area Number |
24580456
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Iwate University (2014)
Hokkaido University (2012-2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Hiroshi 北海道大学, (連合)獣医学研究科, 講師 (50431333)
TAKIGUCHI Mitsuyoshi 北海道大学, (連合)獣医学研究科, 教授 (70261336)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | バベシア症 / Babesia gibsoni / 熱ショックタンパク質90 / クローニング / リアルタイムRT-PCR / ウェスタンブロッティング / 熱ショックタンパク質90阻害薬 / heat shock protein 90 / 原虫増殖 / ストレス応答 / hsp90阻害薬 / 組み替え蛋白質発現 / 抗Bghsp90抗体 / 遺伝子クローニング / 系統樹解析 / 遺伝子発現量解析 |
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| Outline of Final Research Achievements |
In this study, Heat shock protein 90 (Hsp90) in Babesia gibsoni, a blood protozoa in dogs, was analyzed. The Babesia gibsoni heat shock protein 90 (BgHsp90) gene was cloned and sequenced. Moreover, anti recombinant BgHsp90 antibody was developed. Additionally, the expression of BgHsp90 was observed. As results, it was shown that BgHsp90 might contribute the proliferation of the parasites, and that the expression of BgHsp90 would be elevated when the parasites were exposed to the high temperature. Meanwhile, Hsp90 inhibitors could suppress the proliferation of B. gibsoni in vitro. This result suggested that the BgHsp90 should be one of the drug targets for the treatment of canine babesiosis.
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Report
(4 results)
Research Products
(4 results)
