| Project/Area Number |
24580459
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
MORI Takashi 岐阜大学, 応用生物科学部, 准教授 (40402218)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288)
NOGUCHI Shunsuke 山口大学, 獣医学部, 准教授 (10701295)
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| Co-Investigator(Renkei-kenkyūsha) |
AKAO Yukihiro 岐阜大学, 連合創薬医薬医療情報研究科, 教授 (00222505)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | メラノーマ / マイクロRNA / miRNA / イヌ口腔内メラノーマ |
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| Outline of Final Research Achievements |
We demonstrated the antitumor effect in vitro and in vivo of miR-205 that was also chemically modified by benzene-pyridine and had altered passenger sequence. In in vitro experiments, transfection with the synthetic miR-205 (miR-205BP) significantly inhibited the growth of human melanoma cells. On the basis of the results of a luciferase activity assay, miR-205BP directly targeted E2F1, as did Pre-miR-205. However, miR-205BP was much more resistant to RNase than Pre-miR-205 in fetal bovine serum and to RNase in mice xenografted with human melanoma tissues. In addition, the intratumoral injection of miR-205BP exhibited a significant anti- tumor effect compared with the case of control miRNA or Pre-miR-205 in human melanoma cell-xenografted mice.
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