Therapeutic application of microRNA for canine oral malignant melanoma.
Project/Area Number |
24580459
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Clinical veterinary science
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Research Institution | Gifu University |
Principal Investigator |
MORI Takashi 岐阜大学, 応用生物科学部, 准教授 (40402218)
|
Co-Investigator(Kenkyū-buntansha) |
SAKAI Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288)
NOGUCHI Shunsuke 山口大学, 獣医学部, 准教授 (10701295)
|
Co-Investigator(Renkei-kenkyūsha) |
AKAO Yukihiro 岐阜大学, 連合創薬医薬医療情報研究科, 教授 (00222505)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | メラノーマ / マイクロRNA / miRNA / イヌ口腔内メラノーマ |
Outline of Final Research Achievements |
We demonstrated the antitumor effect in vitro and in vivo of miR-205 that was also chemically modified by benzene-pyridine and had altered passenger sequence. In in vitro experiments, transfection with the synthetic miR-205 (miR-205BP) significantly inhibited the growth of human melanoma cells. On the basis of the results of a luciferase activity assay, miR-205BP directly targeted E2F1, as did Pre-miR-205. However, miR-205BP was much more resistant to RNase than Pre-miR-205 in fetal bovine serum and to RNase in mice xenografted with human melanoma tissues. In addition, the intratumoral injection of miR-205BP exhibited a significant anti- tumor effect compared with the case of control miRNA or Pre-miR-205 in human melanoma cell-xenografted mice.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Chemically Modified Synthetic microRNA-205 Inhibits the Growth of Melanoma Cells In Vitro and In Vivo.2013
Author(s)
Noguchi, S., Iwasaki, J., Kumazaki, M., Mori, T., Maruo, K., Saka,i H., Yamada, N., Shimada, K., Naoe, T., Kitade, Y., and Akao, Y.
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Journal Title
Mol. Ther.
Volume: 21
Issue: 6
Pages: 1204-1211
DOI
Related Report
Peer Reviewed
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