Translational research on modified glycosylation model for acute phase protein in severe disease conditions

• Project/Area Number: 24580461
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: IWATA Hiroyuki 山口大学, 獣医学部, 教授 (40193750)
• Co-Investigator(Kenkyū-buntansha): MAEDA Ken 山口大学, 共同獣医学部, 教授 (90284273) ; WATANABE Rie 山口大学, 共同獣医学部, 准教授 (50435715)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 急性期蛋白 / α1酸性糖蛋白 / 糖鎖修飾 / モノクローナル抗体 / 炎症モデル / 組換え蛋白 / 糖鎖 / 動物 / 急性期糖蛋白

Outline of Final Research Achievements

For the purpose of translational research on modified glycosylation model for acute phase protein in severe disease conditions, native alpha-1 acid glycoproteins (AGPs) in animals were purified and recombinant AGPs were also expressed in bacteria and purified. These proteins were used for the preparation of monoclonal antibodies (Mabs) and several Mabs for each animal were recognized with different epitopes for each AGP. In murine, enzyme linked immunoassay system for AGP was developed and its serum levels were increased and its glycan chains were modified on lectin affinity analysis. Furthermore, different excretion patterns of recombinant AGPs which have altered glycan chains were observed on expression in mammalian cells, suggesting that this expression models might be a useful tool for the analyses of glycan chain modification.

Research Products

• [Journal Article] Ayadaulin, a novel RGD peptide with dual antihemostatic activities from the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis (2015)
  • Author(s): Kato, H., Gomez, E.L., Fujita, M., Ishimaru, Y., Uezato, H., Mimori, T., Iwata, H., Hashiguchi, Y.
  • Journal Title: Biochimie Volume: 112 Pages: 49-56
  • DOI: 10.1016/j.biochi.2015.02.011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The host specific NS3 glycosylation pattern reflects the virulence of Ibaraki virus in different hosts (2014)
  • Author(s): Urata M, Watanabe R, Iwata H
  • Journal Title: Virus Res Volume: 181 Pages: 6-10
  • DOI: 10.1016/j.virusres.2013.12.027
  • Peer Reviewed
• [Journal Article] Genetic diversity of the mitochondrial cytochrome b gene in Lutzomyia spp., with special reference to Lutzomyia peruensis, a main vector of Leishmania (Viannia) peruviana in the Peruvian Andes (2013)
  • Author(s): Yamamoto K, Caceres AG, Gomez EA, Mimori T, Iwata H, Korenaga M, Sakurai T, Katakura K, Hashiguchi Y, Kato H
  • Journal Title: Acta Trop Volume: 126 Issue: 2 Pages: 156-163
  • DOI: 10.1016/j.actatropica.2013.02.007
  • NAID: 120005228382
  • Peer Reviewed
• [Journal Article] Prevalence and epidemiological relationship of CMY-2 AmpC β-lactamase and CTX-M extended-spectrum β-lactamase-producing Escherichia coli isolates from broiler farms in Japan (2013)
  • Author(s): Kameyama M, Chuma T, Yabata J, Tominaga K, Iwata H, Okamoto K
  • Journal Title: J Vet Med Sci Volume: 75 Pages: 1009-1015
  • NAID: 130003362165
  • Peer Reviewed
• [Journal Article] Emergence of Salmonella enterica serovar infantis harboring IncI1 plasmid with bla(CTX-M-14) in a broiler farm in Japan. (2012)
  • Author(s): Kameyama M, Chuma T, Yokoi T, Yabata J, Tominaga K, Miyasako D, Iwata H, Okamoto K.
  • Journal Title: J Vet Med Sci Volume: 74(9) Pages: 1213-1216
  • NAID: 130001879742
  • Peer Reviewed
• [Journal Article] Feline infectious peritonitis virus with a large deletion in the 5'-terminal region of the spike gene retains its virulence for cats. (2012)
  • Author(s): Terada Y
  • Journal Title: Journal of General Virology Volume: 93(Pt 9): Issue: 9 Pages: 1930-4
  • DOI: 10.1099/vir.0.043992-0
  • Peer Reviewed
• [Journal Article] Dimiconin, a novel coagulation inhibitor from the kissing bug, Triatoma dimidiata, a vector of Chagas disease. (2012)
  • Author(s): Ishimaru Y, Gomez EA, Zhang F, Martini-Robles L, Iwata H, Sakurai T, Katakura K, Hashiguchi Y, Kato H.
  • Journal Title: J Exp Biol Volume: 215(Pt 20) Pages: 3597-3602
  • Peer Reviewed
• [Presentation] サシチョウバエLutzomyia ayacuchensis唾液腺RGDペプチドAyadualinは2つの作用機序で 止血機構を阻害する (2014)
  • Author(s): 加藤 大智、Gomez Eduardo 、藤田 恵、石丸 由佳、岩田 祐之、三森 龍之、 上里 博、橋口 義久
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] 糖鎖付加型イバラキウイルスNS3による宿主細胞傷害性に関する研究 (2014)
  • Author(s): 浦田真帆、渡邊理恵、岩田祐之
  • Organizer: 第29回中四国ウイルス研究会
  • Place of Presentation: 山口大学（山口市）
  • Year and Date: 2014-06-28 – 2014-06-29
• [Presentation] イバラキウイルス感染細胞における糖鎖付加型NS3の発現パターンの検討 (2013)
  • Author(s): 浦田真帆、渡邊理恵、岩田祐之
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] プロイラー農場におけるESBL、AmpC型β-ラクタマーゼ産生大腸菌の分布 (2013)
  • Author(s): 亀山光博、中馬猛久、矢端順子、富永潔、岩田祐之、岡本嘉六
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] 組換えイバラキウイルスVP6を抗原とした抗VP6血清の作製と感染細胞内VP6の検出
  • Author(s): 足立 萌美、渡邊 理恵、岩田 祐之
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）