| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In vivo study, the isoproterenol (ISO) increased cardiac fibrosis and expression of collagen-I mRNA/ protein in rat left ventricle. These responses were inhibited by the simultaneous administration of the aldosterone receptor inhibitor, eplerenone; EPL. In addition, phosphorylation of ERK1/2 induced by ISO was inhibited with the simultaneous administration of EPL. However, gene expression of aldosterone syntheses was neither detected in the left ventricle nor cardiac fibroblast.
In vitro study, the ISO increased expression of collagen-I and phosphorylation of ERK1/2 in the cardiac fibroblasts. ISO-induced these responses were inhibited by the simultaneous treatment of EPL.
These results suggested that the aldosterone receptor participates in the collagen-I synthesis through the adrenergic receptor in the rat left ventricle/cardiac fibroblast.
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