Study on endophthalmitis treatment of nano-particles of ophthalmic drugs: anti-inflammatory effect and its mechanism

• Project/Area Number: 24580464
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Kitasato University
• Principal Investigator: KANAI KAZUTAKA 北里大学, 獣医学部, 准教授 (50281598)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ナノ化物質 / 眼内炎 / 転写因子 / 抗炎症薬 / ぶどう膜炎 / 治療 / ナノ粒子化点眼薬 / ナノ粒子化点眼液 / 眼内炎治療

Outline of Final Research Achievements

We evaluated the anti-inflammatory effect of 0.5% tranilast nanoparticles (TLn) eyedrops on endotoxin-induced uveitis (EIU) in rats and lipopolysaccharide (LPS)-induced inflammatory response and the underlying mechanisms in RAW264.7 cells. The present results demonstrate that the topical instillation of TLn eyedrops suppresses the inflammation in EIU. The possible mechanisms for the effect of TLn may dependent on the ability to inhibit the activation of NF-κB pathway through p-IKK and/or IκB degeneration and association of early HO-1expression and activation protein (AP)-1 pathway through p-JNK and subsequent expression of proinflammatory mediators. These findings suggest that TLn may be promising agent for the management of inflammation including ophthalmologic field.

Research Products

• [Presentation] INHIBITORY EFFECTS OF TOPICAL TRANILAST-NANOSUSPENSIONS ON ENDOTOXIN-INDUCED UVEITIS IN RATS (2013)
  • Author(s): K kanai, K Nohmi, E Hayashi, Y Ito, N Nagai, K Tajima, N Itoh, Y Hori, S Chikazawa, F Hoshi, and S Higuchi
  • Organizer: ACVO (American college of Veterinary Ophthalmologists)
  • Place of Presentation: Puerto Rico (America)